Western Blot (WB)
(NARF Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NARF antibody detected the NARF protein (arrow).)
Rabbit anti-Human NARF Polyclonal Antibody | anti-NARF antibody
NARF Antibody (Center)
Western Blot (WB)
(NARF Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NARF antibody detected the NARF protein (arrow).)
Western Blot (WB)
(NARF Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NARF antibody detected the NARF protein (arrow).)
methylated at their carboxyl-terminal ends. Prenylation was
initially believed to be important only for membrane attachment.
However, another role for prenylation appears to be its importance
in protein-protein interactions. The only nuclear proteins known to
be prenylated in mammalian cells are prelamin A- and B-type lamins.
Prelamin A is farnesylated and carboxymethylated on the cysteine
residue of a carboxyl-terminal CaaX motif. This
post-translationally modified cysteine residue is removed from
prelamin A when it is endoproteolytically processed into mature
lamin A. The protein encoded by this gene binds to the prenylated
prelamin A carboxyl-terminal tail domain. It may be a component of
a prelamin A endoprotease complex. The encoded protein is located
in the nucleus, where it partially colocalizes with the nuclear
lamina. It shares limited sequence similarity with iron-only
bacterial hydrogenases. Alternatively spliced transcript variants
encoding different isoforms have been identified for this gene,
including one with a novel exon that is generated by RNA editing.
Moller-Krull, M., et al. J. Mol. Biol. 382(3):601-609(2008)
Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008) :
Lattanzi, G., et al. J. Cell. Biochem. 102(5):1149-1159(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
NCBI and Uniprot Product Information
NCBI Description
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]
Uniprot Description
NARF: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: nuclear lamina; nuclear lumen; lamin filament; nucleolus; nucleus
Molecular Function: lamin binding
Research Articles on NARF
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Product Notes
The NARF narf (Catalog #AAA9202355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 134-160. The NARF Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NARF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the NARF narf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NARF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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