Rabbit anti-Human NARF Polyclonal Antibody | anti-NARF antibody

NARF, ID (NARF, Nuclear prelamin A recognition factor, Iron-only hydrogenase-like protein 2) (FITC)

Cat. Num. AAA6323817

• Gene Names: NARF; IOP2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NARF; Polyclonal Antibody; ID (NARF; Nuclear prelamin A recognition factor; Iron-only hydrogenase-like protein 2) (FITC); Iron-only hydrogenase-like protein 2; anti-NARF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NARF antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NARF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the Central region of human NARF.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NARF Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NARF antibody detected the NARF protein (arrow).)

Related Product Information for anti-NARF antibody

Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A-and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

Product Categories/Family for anti-NARF antibody

Antibodies; Enzymes

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Moller-Krull, M., et al. J. Mol. Biol. 382(3):601-609(2008). Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008): Lattanzi, G., et al. J. Cell. Biochem. 102(5):1149-1159(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 84452151
• NCBI GeneID: 26502
• NCBI Accession #: NP_001033707.1; NP_001077077.1
• NCBI GenBank Nucleotide #: NM_001038618.2
• UniProt Accession #: Q9UHQ1; Q96AY9; Q9BWC6; A6NCJ3; B3KPX2; K4DI98
• Molecular Weight: 51,156 Da
• NCBI Official Full Name: nuclear prelamin A recognition factor isoform c
• NCBI Official Synonym Full Names: nuclear prelamin A recognition factor
• NCBI Official Symbol: NARF
• NCBI Official Synonym Symbols: IOP2
• NCBI Protein Information: nuclear prelamin A recognition factor; iron-only hydrogenase-like protein 2; prenyl-dependent prelamin A binding protein
• UniProt Protein Name: Nuclear prelamin A recognition factor
• Protein Family: Nuclear prelamin A recognition factor
• UniProt Gene Name: NARF
• UniProt Synonym Gene Names: IOP2
• UniProt Entry Name: NARF_HUMAN