Mouse Neuregulin 4 (NRG4) ELISA Kit | NRG4 elisa kit

Mouse Neuregulin 4 (NRG4) ELISA Kit

Cat. Num. AAA2020412

• Gene Names: NRG4; HRG4
• Reactivity: Mouse
• Synonyms: Neuregulin 4 (NRG4); Mouse Neuregulin 4 (NRG4) ELISA Kit; HRG4; Pro-NRG4; Pro-neuregulin-4; membrane-bound isoform; NRG4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuregulin 4 (NRG4).; No significant cross-reactivity or interference between Neuregulin 4 (NRG4) and analogues was observed.
• Sequence Length: 115

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuregulin 4 (NRG4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuregulin 4 (NRG4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRG4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuregulin 4 (NRG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuregulin 4 (NRG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuregulin 4 (NRG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuregulin 4 (NRG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NRG4 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 17068379
• NCBI GeneID: 145957
• UniProt Accession #: Q8WWG1; A6NIE8
• Molecular Weight: 12,722 Da
• NCBI Official Full Name: Neuregulin 4
• NCBI Official Synonym Full Names: neuregulin 4
• NCBI Official Symbol: NRG4
• NCBI Official Synonym Symbols: HRG4
• NCBI Protein Information: pro-neuregulin-4, membrane-bound isoform
• UniProt Protein Name: Pro-neuregulin-4, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: NRG4
• UniProt Synonym Gene Names: Pro-NRG4; NRG-4
• UniProt Entry Name: NRG4_HUMAN

NCBI Description

The neuregulins, including NRG4, activate type-1 growth factor receptors (see EGFR; MIM 131550) to initiating cell-to-cell signaling through tyrosine phosphorylation (Harari et al., 1999 [PubMed 10348342]).[supplied by OMIM, Mar 2008]

Uniprot Description

NRG4: Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors. Belongs to the neuregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: extracellular space; extracellular region; integral to membrane; plasma membrane

Molecular Function: growth factor activity; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; organ development; innate immune response