Western Blot (WB)
(NARF Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NARF antibody detected the NARF protein (arrow).)
Rabbit anti-Human NARF Polyclonal Antibody | anti-NARF antibody
NARF, ID (NARF, Nuclear prelamin A recognition factor, Iron-only hydrogenase-like protein 2) (APC)
Gene Names
NARF; IOP2
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NARF; Polyclonal Antibody; ID (NARF; Nuclear prelamin A recognition factor; Iron-only hydrogenase-like protein 2) (APC); Iron-only hydrogenase-like protein 2; anti-NARF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-NARF antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
NARF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the Central region of human NARF.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(NARF Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NARF antibody detected the NARF protein (arrow).)
Western Blot (WB)
(NARF Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NARF antibody detected the NARF protein (arrow).)
Related Product Information for anti-NARF antibody
Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A-and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Product Categories/Family for anti-NARF antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Moller-Krull, M., et al. J. Mol. Biol. 382(3):601-609(2008). Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008): Lattanzi, G., et al. J. Cell. Biochem. 102(5):1149-1159(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,156 Da
NCBI Official Full Name
nuclear prelamin A recognition factor isoform c
NCBI Official Synonym Full Names
nuclear prelamin A recognition factor
NCBI Official Symbol
NARF
NCBI Official Synonym Symbols
IOP2
NCBI Protein Information
nuclear prelamin A recognition factor; iron-only hydrogenase-like protein 2; prenyl-dependent prelamin A binding protein
UniProt Protein Name
Nuclear prelamin A recognition factor
Protein Family
UniProt Gene Name
NARF
UniProt Synonym Gene Names
IOP2
UniProt Entry Name
NARF_HUMAN
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Product Notes
The NARF narf (Catalog #AAA6323815) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NARF, ID (NARF, Nuclear prelamin A recognition factor, Iron-only hydrogenase-like protein 2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NARF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NARF narf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NARF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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