Mouse serpin peptidase inhibitor, clade G (C1 inhibitor) , member 1 ELISA Kit | SERPING1 elisa kit

Mouse Plasma protease C1 inhibitor, SERPING1 ELISA Kit

Cat. Num. AAA914924

• Gene Names: Serping1; C1nh; C1INH
• Reactivity: Mouse
• Synonyms: serpin peptidase inhibitor; clade G (C1 inhibitor); member 1; Mouse Plasma protease C1 inhibitor; SERPING1 ELISA Kit; Mouse Plasma protease C1 inhibitor (SERPING1) ELISA kit; C1IN; C1INH; C1NH; HAE1; HAE2; complement component 1 inhibitor; plasma protease C1 inhibitor; serine/cysteine proteinase inhibitor clade G member 1; clade G; SERPING1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SERPING1. No significant cross-reactivity or interference between mouse SERPING1 and analogues was observed.
• Sequence Length: 504

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 5.6 ng/ml -360 ng/ml.
• Sensitivity: The minimum detectable dose of mouse SERPING1 is typically less than 1.4 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERPING1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPING1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPING1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPING1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPING1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 163914390
• NCBI GeneID: 12258
• NCBI Accession #: NP_033906.2
• NCBI GenBank Nucleotide #: NM_009776.3
• UniProt Accession #: P97290; O88330; Q99M43; Q9QX09; A2ATR7
• Molecular Weight: 55,585 Da
• NCBI Official Full Name: plasma protease C1 inhibitor
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade G, member 1
• NCBI Official Symbol: Serping1
• NCBI Official Synonym Symbols: C1nh; C1INH
• NCBI Protein Information: plasma protease C1 inhibitor; C1 Inh; serpin G1; C1-inhibiting factor; C1 esterase inhibitor; complement component 1 inhibitor; serine (or cysteine) proteinase inhibitor, clade G, member 1; serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1
• UniProt Protein Name: Plasma protease C1 inhibitor
• Protein Family: Plasma protease C1 inhibitor
• UniProt Gene Name: Serping1
• UniProt Synonym Gene Names: C1nh; C1 Inh; C1Inh
• UniProt Entry Name: IC1_MOUSE

Uniprot Description

SERPING1: a protein protease inhibitor (C1-inhibitor) that forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play an important role in regulating complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Mutations of the SERPING1 gene is associated with adult macular degeneration can also cause hereditary angioedema. Binds to E.coli stcE which allows localization of SERPING1 to cell membranes thus protecting the bacteria against complement-mediated lysis. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: complement binding; serine-type endopeptidase inhibitor activity; protease inhibitor activity

Biological Process: negative regulation of complement activation, lectin pathway; fibrinolysis; hemostasis; immune system process; negative regulation of complement activation; regulation of blood coagulation; negative regulation of peptidase activity; innate immune response; blood coagulation; complement activation, classical pathway