Typical Testing Data/Standard Curve (for reference only)
Human serpin peptidase inhibitor, clade B (ovalbumin) , member 1 ELISA Kit | SERPINB1 elisa kit
Human Leukocyte elastase inhibitor, SERPINB1 ELISA Kit
Gene Names
SERPINB1; EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2
Reactivity
Human
Synonyms
serpin peptidase inhibitor; clade B (ovalbumin); member 1; Human Leukocyte elastase inhibitor; SERPINB1 ELISA Kit; Human Leukocyte elastase inhibitor (SERPINB1) ELISA kit; EI; ELANH2; LEI; M/NEI; MNEI; PI2; anti-elastase; protease inhibitor 2 (anti-elastase); monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor; SERPINB1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human SERPINB1. No significant cross-reactivity or interference between human SERPINB1 and analogues was observed.
Sequence Length
379
Samples
Serum, plasma, tissue homogenates and Cell lysates
Assay Type
Sandwich
Detection Range
0.6 25 ng/ml -40 ng/ml.
Sensitivity
The minimum detectable dose of human SERPINB1 is typically less than 0.156 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SERPINB1 elisa kit
Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,742 Da
NCBI Official Full Name
leukocyte elastase inhibitor
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 1
NCBI Official Symbol
SERPINB1
NCBI Official Synonym Symbols
EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2
NCBI Protein Information
leukocyte elastase inhibitor; peptidase inhibitor 2; epididymis luminal protein 57; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1
UniProt Protein Name
Leukocyte elastase inhibitor
Protein Family
UniProt Gene Name
SERPINB1
UniProt Synonym Gene Names
ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2
UniProt Entry Name
ILEU_HUMAN
NCBI Description
The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
SERPINB1: Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Belongs to the serpin family. Ov-serpin subfamily.
Chromosomal Location of Human Ortholog: 6p25
Cellular Component: extracellular space; membrane; cytoplasm
Molecular Function: serine-type endopeptidase inhibitor activity
Research Articles on SERPINB1
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Product Notes
The Human SERPINB1 serpinb1 (Catalog #AAA921070) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA921070 ELISA Kit recognizes Human SERPINB1. It is sometimes possible for the material contained within the vial of "serpin peptidase inhibitor, clade B (ovalbumin) , member 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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