Human serpin peptidase inhibitor, clade C (antithrombin) , member 1 ELISA Kit | SERPINC1 elisa kit

Human Antithrombin III, AT III ELISA Kit

Cat. Num. AAA944553

• Gene Names: SERPINC1; AT3; AT3D; ATIII; THPH7
• Reactivity: Human
• Synonyms: serpin peptidase inhibitor; clade C (antithrombin); member 1; Human Antithrombin III; AT III ELISA Kit; Human Antithrombin III (AT III) ELISA Kit; AT3; ATIII; MGC22579; antithrombin III; serine (or cysteine) proteinase inhibitor; serine-cysteine proteinase inhibitor clade C member 1; c; SERPINC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human AT III. No significant cross-reactivity or interference between human AT III and analogues was observed.
• Sequence Length: 464

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 9.38 ng/ml -600 ng/ml.
• Sensitivity: The minimum detectable dose of human AT III is typically less than 2.34 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SERPINC1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AT III has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AT III present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for AT III is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AT III bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4502261
• NCBI GeneID: 462
• NCBI Accession #: NP_000479.1
• NCBI GenBank Nucleotide #: NM_000488.3
• UniProt Accession #: P01008; P78439; P78447; Q13815; Q5TC78; Q7KZ43; Q7KZ97; Q9UC78; B2R6P0
• Molecular Weight: 52,602 Da
• NCBI Official Full Name: antithrombin-III
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade C (antithrombin), member 1
• NCBI Official Symbol: SERPINC1
• NCBI Official Synonym Symbols: AT3; AT3D; ATIII; THPH7
• NCBI Protein Information: antithrombin-III; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• UniProt Protein Name: Antithrombin-III
• Protein Family: Antithrombin
• UniProt Gene Name: SERPINC1
• UniProt Synonym Gene Names: AT3; PRO0309; ATIII
• UniProt Entry Name: ANT3_HUMAN

NCBI Description

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]

Uniprot Description

SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; heparin binding; protein binding; protease binding

Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient

Disease: Antithrombin Iii Deficiency