Rabbit anti-Mouse, Rat PDE12 Polyclonal Antibody | anti-PDE12 antibody

PDE12 Rabbit pAb

Cat. Num. AAA9142691

• Gene Names: PDE12; 2-PDE; 2'-PDE
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity purification
• Synonyms: PDE12; Polyclonal Antibody; PDE12 Rabbit pAb; 2-PDE; anti-PDE12 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Sequence: AVEKLSRAEAGSQTAAGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKSRPNASGGAACSGPGPEPAVFCEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSY

• Applicable Applications for anti-PDE12 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-300 of human PDE12 (NP_001309106.1).
• Positive Samples: Mouse thymus, Rat thymus
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PDE12 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of C6 cells using PDE12 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH/3T3 cells using PDE12 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 189027129
• NCBI GeneID: 201626
• NCBI Accession #: NP_808881.3
• NCBI GenBank Nucleotide #: NM_177966.6
• UniProt Accession #: Q6L8Q7; Q8IYU3; Q8NDU2; Q8TE78; B4DTU8
• Molecular Weight: 58,890 Da
• NCBI Official Full Name: 2',5'-phosphodiesterase 12 isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 12
• NCBI Official Symbol: PDE12
• NCBI Official Synonym Symbols: 2-PDE; 2'-PDE
• NCBI Protein Information: 2',5'-phosphodiesterase 12
• UniProt Protein Name: 2',5'-phosphodiesterase 12
• Protein Family: 2',5'-phosphodiesterase
• UniProt Gene Name: PDE12
• UniProt Synonym Gene Names: 2'-PDE; 2-PDE

Uniprot Description

Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.