Rat Cluster of Differentiation 200 ELISA Kit | CD200 elisa kit

Rat Cluster of Differentiation 200 ELISA Kit

Cat. Num. AAA726520

• Gene Names: CCL15; LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
• Reactivity: Rat
• Synonyms: Cluster of Differentiation 200; Rat Cluster of Differentiation 200 ELISA Kit; CD200 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CD200 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CD200. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD200 elisa kit

Principle of the assay: CD200 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD200 antibody and an CD200-HRP conjugate. The assay sample and buffer are incubated together with CD200-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD200 concentration since CD200 from samples and CD200-HRP conjugate compete for the anti-CD200 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD200 from the sample, fewer sites are left to bind CD200-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD200 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CD200 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4204910
• NCBI GeneID: 6359
• Molecular Weight: 12,248 Da
• NCBI Official Full Name: chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 15
• NCBI Official Symbol: CCL15
• NCBI Official Synonym Symbols: LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
• NCBI Protein Information: C-C motif chemokine 15; leukotactin 1; chemokine CC-2; new CC chemokine 3; small-inducible cytokine A15; macrophage inflammatory protein 5; small inducible cytokine subfamily A (Cys-Cys), member 15
• UniProt Protein Name: C-C motif chemokine 15
• Protein Family: OX-2 membrane glycoprotein
• UniProt Gene Name: CCL15
• UniProt Synonym Gene Names: MIP5; NCC3; SCYA15; HCC-2; LKN-1; MIP-5
• UniProt Entry Name: CCL15_HUMAN

NCBI Description

This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]

Uniprot Description

CCL15: Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space

Molecular Function: heparin binding; chemokine activity; receptor binding; chemoattractant activity

Biological Process: cellular calcium ion homeostasis; cell-cell signaling; positive chemotaxis; immune response; signal transduction; chemotaxis