Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein sidekick-1 (SDK1) Standard Curve.)
Human Sidekick-1 (SDK1) ELISA Kit | SDK1 elisa kit
Human Protein Sidekick-1 (SDK1) ELISA Kit
Reactivity
Human
Synonyms
Sidekick-1 (SDK1); Human Protein Sidekick-1 (SDK1) ELISA Kit; SDK1; FLJ31425; sidekick 1; SDK1 elisa kit
Reactivity
Human
Specificity
Human Protein sidekick-1 (SDK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SDK1. No significant cross-reactivity or interference between Human SDK1 and analogues was observed.
Sequence Length
2213
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein sidekick-1 (SDK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein sidekick-1 (SDK1) Standard Curve.)
Related Product Information for SDK1 elisa kit
Description: This Human Protein sidekick-1 (SDK1) ELISA Kit employs a two-site sandwich ELISA to quantitate SDK1 in samples. An antibody specific for SDK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.
Background: The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein sidekick-1 isoform 1
NCBI Official Synonym Full Names
sidekick cell adhesion molecule 1
NCBI Official Symbol
SDK1
NCBI Protein Information
protein sidekick-1
UniProt Protein Name
Protein sidekick-1
Protein Family
UniProt Gene Name
SDK1
UniProt Entry Name
SDK1_HUMAN
NCBI Description
The protein encoded by this gene is a member of the immunoglobulin superfamily. The protein contains six immunoglobulin-like domains and thirteen fibronectin type III domains. Fibronectin type III domains are present in both extracellular and intracellular proteins and tandem repeats are known to contain binding sites for DNA, heparin and the cell surface. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]
Research Articles on SDK1
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Product Notes
The Human SDK1 sdk1 (Catalog #AAA9714390) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714390 ELISA Kit recognizes Human SDK1. It is sometimes possible for the material contained within the vial of "Sidekick-1 (SDK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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