Human Sidekick-1 (SDK1) ELISA Kit | SDK1 elisa kit

Human Protein Sidekick-1 (SDK1) ELISA Kit

Cat. Num. AAA9714390

• Reactivity: Human
• Synonyms: Sidekick-1 (SDK1); Human Protein Sidekick-1 (SDK1) ELISA Kit; SDK1; FLJ31425; sidekick 1; SDK1 elisa kit

• Reactivity: Human
• Specificity: Human Protein sidekick-1 (SDK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SDK1. No significant cross-reactivity or interference between Human SDK1 and analogues was observed.
• Sequence Length: 2213

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Protein sidekick-1 (SDK1) Standard Curve.)

Related Product Information for SDK1 elisa kit

Description: This Human Protein sidekick-1 (SDK1) ELISA Kit employs a two-site sandwich ELISA to quantitate SDK1 in samples. An antibody specific for SDK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog.

NCBI and Uniprot Product Information

• NCBI GI #: 119220552
• NCBI GeneID: 221935
• NCBI Accession #: NP_689957.3
• NCBI GenBank Nucleotide #: NP_689957.3
• UniProt Accession #: Q7Z5N4
• NCBI Official Full Name: protein sidekick-1 isoform 1
• NCBI Official Synonym Full Names: sidekick cell adhesion molecule 1
• NCBI Official Symbol: SDK1
• NCBI Protein Information: protein sidekick-1
• UniProt Protein Name: Protein sidekick-1
• Protein Family: Protein sidekick
• UniProt Gene Name: SDK1
• UniProt Entry Name: SDK1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin superfamily. The protein contains six immunoglobulin-like domains and thirteen fibronectin type III domains. Fibronectin type III domains are present in both extracellular and intracellular proteins and tandem repeats are known to contain binding sites for DNA, heparin and the cell surface. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]