Rabbit anti-Human PDE12 Polyclonal Antibody | anti-PDE12 antibody

PDE12, CT (PDE12, 2',5'-phosphodiesterase 12) (FITC)

Cat. Num. AAA6332081

• Gene Names: PDE12; 2-PDE; 2'-PDE
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDE12; Polyclonal Antibody; CT (PDE12; 2'; 5'-phosphodiesterase 12) (FITC); 5'-phosphodiesterase 12; anti-PDE12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PDE12 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PDE12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 369-398 amino acids from the C-terminal region of human PDE12.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDE12 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PDE12 antibody detected the PDE12 protein (arrow).)

Flow Cytometry (FC/FACS)

(PDE12 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PDE12 antibody

Antibodies; Enzymes; Phosphodiesterase

References

Kohno, T., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 5), 520-522 (2010): Kubota, K., et al. J. Biol. Chem. 279(36):37832-37841(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 189027129
• NCBI GeneID: 201626
• NCBI Accession #: NP_808881.3
• NCBI GenBank Nucleotide #: NM_177966.6
• UniProt Accession #: Q6L8Q7; Q8IYU3; Q8NDU2; Q8TE78; B4DTU8
• Molecular Weight: 58,890 Da
• NCBI Official Full Name: 2',5'-phosphodiesterase 12 isoform 1
• NCBI Official Synonym Full Names: phosphodiesterase 12
• NCBI Official Symbol: PDE12
• NCBI Official Synonym Symbols: 2-PDE; 2'-PDE
• NCBI Protein Information: 2',5'-phosphodiesterase 12
• UniProt Protein Name: 2',5'-phosphodiesterase 12
• Protein Family: 2',5'-phosphodiesterase
• UniProt Gene Name: PDE12
• UniProt Synonym Gene Names: 2'-PDE; 2-PDE

Uniprot Description

Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.