Human IL-2 ELISA Kit | IL-2 elisa kit

Human IL-2 ELISA Kit

Cat. Num. AAA9141982

• Reactivity: Human
• Synonyms: IL-2; Human IL-2 ELISA Kit; IL-2 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes both recombinant and natural human IL-2. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.

• Samples: Cell culture supernates, serum and plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: < 3.9 pg/mL
• Intra-assay Precision: Three samples of known concentration were tested 20 times on one plate to evaluate the Intra-plate precision.
• Inter-assay Precision: Three samples of known concentration were tested 20 times separate assays to evaluate the Inter-plate precision.Assay were using two lots of components.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL-2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-2 is added to the wells and binds to the combination of capture antibody-IL-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of IL-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven IL-2 standard dilutions and IL-2 sample concentration determined.

Background/Introduction: Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha-helical polypeptide that is a member of the Common gamma Chain (gammac) cytokine family (1-4). It exists as a monomer and has a notably short half-life (< 30 minutes) (1). Human IL-2 is synthesized as a 153 amino acid precursor that contains a 20 aa signal sequence plus a 133 aa mature region (5, 6). The mature region is alpha-helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity (7). Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gammadelta T cells (8), activated conventional CD4+ and CD8+ T cells (1, 9), neurons (10, 11), microglia (12), and hematopoietic stem cells (13). The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 Ralpha chain, the 70 kDa IL-2 Rbeta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 Rbeta and gammac to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 Rbeta is used by IL-15 in its quaternary signaling complex. gammac also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3). In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).