Mouse GM-CSF ELISA Kit | GM-CSF elisa kit

Mouse GM-CSF ELISA Kit

Cat. Num. AAA9135768

• Gene Names: CSF2; CSF; GMCSF
• Reactivity: Mouse
• Synonyms: GM-CSF; Mouse GM-CSF ELISA Kit; GM-CSF elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes both recombinant and natural mouse GM-CSF. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity. No significant cross-reactivity was observed with the following: Recombinant mouse Recombinant human IL-10 G-CSF C10 GM-CSF IL-13 M-CSF G-CSF JE IL-1alpha LIF IL-1beta M-CSF IL-2 MIP-1alpha IL-3 MIP-1beta IL-4 MIP-2 IL-5 SCF IL-6 TNF-alpha IL-7 IL-9
• Sequence Length: 144

• Samples: Cell culture supernates, serum and plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: < 7.9 pg/mL
• Intra-assay Precision: Three samples of known concentration were tested 20 times on one plate to evaluate the Intra-plate precision.
• Inter-assay Precision: Three samples of known concentration were tested 20 times separate assays to evaluate the Inter-plate precision.Assay were using two lots of components.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GM-CSF elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for GM-CSF is added to the wells and binds to the combination of capture antibody-GM-CSF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of GM-CSF present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven GM-CSF standard dilutions and GM-CSF sample concentration determined.

Background/Introduction: Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), also known as CSF-2, is a pleiotrophic 30 kDa member of the Common beta Chain (betac) cytokine family that also includes IL-3 and IL-5. GM-CSF adopts an alpha-helical configuration with two intrachain disulfide bonds. It is secreted by a wide variety of activated immune, mesenchymal, and epithelial cell types and circulates as a variably glycosylated monomer (1, 2). It is upregulated in multiple cell types during inflammation including encephalitogenic T cells (3-5), allergen exposed lung endothelial cells (6), and IgE activated mast cells (7). Mature mouse GM-CSF shares 54% and 69% amino acid sequence identity with human and rat GM-CSF, respectively (8). Rat GM-CSF is known to be active on mouse cells, while mouse GMCSF has limited activity on rat cells (9). The high-affinity receptor for GM-CSF is composed of a 50 kDa ligand binding alpha subunit (GM-CSF Ralpha) (10) and a 120 kDa signal transducing Common beta Chain (betac) (11, 12). The stoichiometry for the functional GM-CSF receptor is reported to be a 2:2:2 complex of GM-CSF, GM-CSF Ralpha, and betac (13, 14). Notably, the betac subunit is shared by the receptor complexes for IL-3 and IL-5, and IL-5 may signal through GM-CSF Ralpha and betac (14). GM-CSF may additionally utilize Syndecan-2 as a coreceptor (15). A number of functions have been attributed to GM-CSF. It induces monocyte, neutrophil, and eosinophil production from CD34 + stem cell precursors (16, 17). It can act in concert with IL-4 or Flt-3 Ligand to induce the development and maintenance of myeloid and dermal dendritic cells (17-21). It also acts as a neutrophil and dendritic cell chemoattractant (6, 22). GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils (3-5, 23-27). In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion (28).

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: CSF; GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.