Typical Testing Data/Standard Curve (for reference only)
Mouse CXCL1/KC ELISA Kit | CXCL1 elisa kit
Mouse CXCL1/KC ELISA Kit
Reactivity
Mouse
Synonyms
CXCL1/KC; Mouse CXCL1/KC ELISA Kit; CXCL1 elisa kit
Reactivity
Mouse
Specificity
This assay recognizes both recombinant and natural mouse CXCL1/KC. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
Sequence Length
107
Samples
Cell culture supernates, serum and plasma
Assay Type
Quantitative Sandwich
Intra-assay Precision
Three samples of known concentration were tested 20 times on one plate to evaluate the Intra-plate precision.
Inter-assay Precision
Three samples of known concentration were tested 20 times separate assays to evaluate the Inter-plate precision.Assay were using two lots of components.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CXCL1/KC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL1/KC present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for CXCL1/KC is added to the wells and binds to the combination of capture antibody-CXCL1/KC in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of CXCL1/KC present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven CXCL1/KC standard dilutions and CXCL1/KC sample concentration determined.!!Background/Introduction: CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
NCBI Official Symbol
CXCL1
NCBI Protein Information
chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Protein Family
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Product Notes
The Mouse CXCL1 (Catalog #AAA9135758) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9135758 ELISA Kit recognizes Mouse CXCL1. It is sometimes possible for the material contained within the vial of "CXCL1/KC, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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