Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GSDMD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Rabbit GSDMD Polyclonal Antibody | anti-GSDMD antibody
GSDMD Polyclonal Antibody
Gene Names
Gsdmd; DF5L; M2-4; Dfna5l; Gsdmdc1; AW558049; 1810036L03Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Synonyms
GSDMD; Polyclonal Antibody; GSDMD Polyclonal Antibody; 1810036L03Rik; AW558049; DF5L; Dfna5l; Gsdmdc1; M2-4; anti-GSDMD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
487
Applicable Applications for anti-GSDMD antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IF : 1:50-1:200
IF : 1:50-1:200
Samples
Urine, Serum, Plasma, Tissue Extracts, Cell Lysate, Cell Culture Media, Other Biological Fluids Samples
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GSDMD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GSDMD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Related Product Information for anti-GSDMD antibody
Background/Introduction: Gamma-glutamyltransferase (also gamma-glutamyltransferase, GGT, gamma-GT; EC 2.3.2.2) is a transferase (a type of enzyme) that catalyzes the transfer of gamma-glutamyl functional groups from molecules such as glutathione to an acceptor that may be an amino acid, a peptide or water (forming glutamate). GGT plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione and drug and xenobiotic detoxification. Other lines of evidence indicate that GGT can also exert a pro-oxidant role, with regulatory effects at various levels in cellular signal transduction and cellular pathophysiology. This transferase is found in many tissues, the most notable one being the liver, and has significance in medicine as a marker. Gamma-glutamyltransferase Microplate Assay Kit is a sensitive assay for determining Gamma-glutamyltransferase activity in various samples. Gamma-glutamyltransferase activity is determined by the product of paranitroaniline. The reaction products can be measured at a colorimetric readout at 405 nm.
Product Categories/Family for anti-GSDMD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Gasdermin-D
NCBI Official Synonym Full Names
gasdermin D
NCBI Official Symbol
Gsdmd
NCBI Official Synonym Symbols
DF5L; M2-4; Dfna5l; Gsdmdc1; AW558049; 1810036L03Rik
NCBI Protein Information
gasdermin-D
UniProt Protein Name
Gasdermin-D
Protein Family
UniProt Gene Name
Gsdmdc1
UniProt Synonym Gene Names
Gsdmd; GSDMD-CT
Uniprot Description
FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 15|15 D3
Cellular Component: cytosol; extracellular space; nucleoplasm; plasma membrane
Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding
Biological Process: cellular response to extracellular stimulus; cytolysis; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; pore complex biogenesis; positive regulation of interleukin-1 beta secretion; protein homooligomerization
Research Articles on GSDMD
Similar Products
Product Notes
The GSDMD gsdmdc1 (Catalog #AAA9130687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSDMD Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSDMD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500 - 1:2000 IF : 1:50-1:200. Researchers should empirically determine the suitability of the GSDMD gsdmdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSDMD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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