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SDS-Page (Figure 1. Human 2B4, hFc Tag on SDS-PAGE under reducing condition.)

2B4 recombinant protein

Recombinant human 2B4 protein with C-terminal human Fc tag

Gene Names
CD244; 2B4; NAIL; Nmrk; NKR2B4; SLAMF4
Applications
Western Blot, ELISA
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
2B4; Recombinant human 2B4 protein with C-terminal human Fc tag; Human 2B4 Protein; hFc tag; CD244; SLAMF4; NKR2B4; NAIL; h2B4; 2B4 recombinant protein
Ordering
For Research Use Only!
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-96% trehalose is added as protectants before lyophilization.
Sequence
2B4(Cys22-Ala221)+hFc(Glu99-Ala330)
Applicable Applications for 2B4 recombinant protein
Western Blot (WB), ELISA (EIA)
Source
Human
Conjugation
C-Human Fc tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice

SDS-Page

(Figure 1. Human 2B4, hFc Tag on SDS-PAGE under reducing condition.)

SDS-Page (Figure 1. Human 2B4, hFc Tag on SDS-PAGE under reducing condition.)

ELISA (EIA)

(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD48, mFc-His tagged protein can bind Human 2B4, hFc tagged protein in a linear range of 62.5-4000 ng/ml.)

ELISA (EIA) (Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD48, mFc-His tagged protein can bind Human 2B4, hFc tagged protein in a linear range of 62.5-4000 ng/ml.)
Related Product Information for 2B4 recombinant protein
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
370
NCBI Official Full Name
Natural killer cell receptor 2B4
NCBI Official Synonym Full Names
CD244 molecule, natural killer cell receptor 2B4
NCBI Official Symbol
CD244
NCBI Official Synonym Symbols
2B4; NAIL; Nmrk; NKR2B4; SLAMF4
NCBI Protein Information
natural killer cell receptor 2B4; h2B4; SLAM family member 4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL; signaling lymphocytic activation molecule 4
UniProt Protein Name
Natural killer cell receptor 2B4
UniProt Gene Name
CD244
UniProt Synonym Gene Names
2B4; NAIL; NKR2B4; h2B4; SLAMF4
UniProt Entry Name
CD244_HUMAN

NCBI Description

This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

CD244: Modulate other receptor-ligand interactions to enhance leukocyte activation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: signal transduction; blood coagulation; leukocyte migration

Disease: Rheumatoid Arthritis

Research Articles on 2B4

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Product Notes

The 2B4 cd244 (Catalog #AAA188156) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 2B4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the 2B4 cd244 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 2B4(Cys22- Ala221)+hF c(Glu99-Al a330). It is sometimes possible for the material contained within the vial of "2B4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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