Western Blot (WB)
(Western blot analysis of extracts of RAW264.7 cells, using GSDMD (Full Length+N terminal) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
Rabbit GSDMD Polyclonal Antibody | anti-GSDMD antibody
GSDMD (Full Length+N terminal) Rabbit pAb
IF: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts of RAW264.7 cells, using GSDMD (Full Length+N terminal) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
Western Blot (WB)
(Western blot analysis of extracts of RAW264.7 cells, using GSDMD (Full Length+N terminal) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.)
Western Blot (WB)
(Western blot analysis of extracts of THP-1 cells, using GSDMD (Full Length+N terminal) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of THP-1 cells, using GSDMD (Full Length+N terminal) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of PC-12 cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of PC-12 cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
Uniprot Description
FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 8q24.3
Biological Process: cellular response to extracellular stimulus
Research Articles on GSDMD
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Product Notes
The GSDMD gsdmd (Catalog #AAA9143598) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GSDMD (Full Length+N terminal) Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSDMD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the GSDMD gsdmd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSDMD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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