Rabbit anti-Human, Mouse GSDMD Polyclonal Antibody | anti-GSDMD antibody

GSDMD (Full Length+C terminal) Rabbit pAb

Cat. Num. AAA9142706

• Gene Names: GSDMD; DF5L; DFNA5L; FKSG10; GSDMDC1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: GSDMD; Polyclonal Antibody; GSDMD (Full Length+C terminal) Rabbit pAb; DF5L; DFNA5L; FKSG10; GSDMDC1; anti-GSDMD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-GSDMD antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Customer Validation: WB: Human
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human GSDMD (NP_079012.3).
• Positive Samples: PC-3, THP-1, RAW264.7
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GSDMD antibody at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 ug/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

Western Blot (WB)

(Western blot analysis of extracts of RAW264.7 cells, using GSDMD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of NIH/3T3 cells using GSDMD (Full Length+C terminal) Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of PC-12 cells using GSDMD (Full Length+C terminal) Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using GSDMD (Full Length+C terminal) Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.)

Related Product Information for anti-GSDMD antibody

Background: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-GSDMD antibody

Immunology & Inflammation; Inflammasome Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 83320070
• NCBI GeneID: 79792
• NCBI Accession #: NP_079012.3
• NCBI GenBank Nucleotide #: NM_024736.6
• UniProt Accession #: P57764; Q96Q98; D3DWJ9
• Molecular Weight: 52,801 Da
• NCBI Official Full Name: gasdermin-D
• NCBI Official Synonym Full Names: gasdermin D
• NCBI Official Symbol: GSDMD
• NCBI Official Synonym Symbols: DF5L; DFNA5L; FKSG10; GSDMDC1
• NCBI Protein Information: gasdermin-D; gasdermin domain containing 1; gasdermin domain-containing protein 1
• UniProt Protein Name: Gasdermin-D
• Protein Family: Gasdermin
• UniProt Gene Name: GSDMD
• UniProt Synonym Gene Names: DFNA5L; GSDMDC1
• UniProt Entry Name: GSDMD_HUMAN

NCBI Description

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Uniprot Description

FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q24.3

Biological Process: cellular response to extracellular stimulus