Human stem-loop binding protein ELISA Kit | SLBP elisa kit

Human Histone RNA hairpin-binding protein, SLBP ELISA Kit

Cat. Num. AAA904587

• Gene Names: SLBP; HBP
• Reactivity: Human
• Synonyms: stem-loop binding protein; Human Histone RNA hairpin-binding protein; SLBP ELISA Kit; Human Histone RNA hairpin-binding protein (SLBP) ELISA kit; HBP; hairpin binding protein; histone; histone binding protein; histone stem-loop binding protein; stem-loop (histone) binding protein; SLBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SLBP. No significant cross-reactivity or interference between human SLBP and analogues was observed.
• Sequence Length: 270

• Samples: Serum, plasma, tissue homogenates, cell lysates.
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: 3.9 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLBP elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SLBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5729862
• NCBI GeneID: 7884
• NCBI Accession #: NP_006518.1
• NCBI GenBank Nucleotide #: NM_006527.2
• UniProt Accession #: Q14493; B3KRJ5
• Molecular Weight: 31,286 Da
• NCBI Official Full Name: histone RNA hairpin-binding protein
• NCBI Official Synonym Full Names: stem-loop binding protein
• NCBI Official Symbol: SLBP
• NCBI Official Synonym Symbols: HBP
• NCBI Protein Information: histone RNA hairpin-binding protein; histone binding protein; hairpin binding protein, histone; histone stem-loop binding protein; histone stem-loop-binding protein; stem-loop (histone) binding protein
• UniProt Protein Name: Histone RNA hairpin-binding protein
• UniProt Gene Name: SLBP
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: SLBP_HUMAN

NCBI Description

This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq, Jul 2008]

Uniprot Description

SLBP: RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. Binds to the 5' side of the stem-loop structure of histone pre-mRNAs. Monomer. SLBP/pre-mRNA complex interacts with ZNF473. Interacts with the Importin alpha/Importin beta receptor, LSM1, MIF4GD, TNPO3 and UPF1. Interaction with LSM1 occurs when histone mRNA is being rapidly degraded during the S phase. Found in a ternary complex with ERI1 and the stem-loop structure of the 3' end of histone mRNA. Associates with polyribosomes. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Binds in a cooperative manner with ERI1 to the mature 3'-end of histone mRNAs. Widely expressed. Belongs to the SLBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: mRNA binding; protein binding

Biological Process: transcription from RNA polymerase II promoter; mRNA export from nucleus; mRNA transport; histone mRNA metabolic process; DNA replication during S phase; histone mRNA 3'-end processing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription