Human interferon, kappa ELISA Kit | IFNK elisa kit

Human Interferon kappa, IFNK ELISA Kit

Cat. Num. AAA936153

• Gene Names: IFNK; IFNT1; INFE1
• Reactivity: Human
• Synonyms: interferon; kappa; Human Interferon kappa; IFNK ELISA Kit; Human Interferon kappa (IFNK) ELISA kit; RP11-27J8.1; interferon kappa; interferon-like protein; IFNK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IFNK. No significant cross-reactivity or interference between human IFNK and analogues was observed.
• Sequence Length: 207

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFNK elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFNK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNK bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 112789564
• NCBI GeneID: 56832
• NCBI Accession #: NP_064509.2
• NCBI GenBank Nucleotide #: NM_020124.2
• UniProt Accession #: Q9P0W0; Q5T166
• Molecular Weight: 25,218 Da
• NCBI Official Full Name: interferon kappa
• NCBI Official Synonym Full Names: interferon, kappa
• NCBI Official Symbol: IFNK
• NCBI Official Synonym Symbols: IFNT1; INFE1
• NCBI Protein Information: interferon kappa; IFN-kappa; interferon-like protein
• UniProt Protein Name: Interferon kappa
• Protein Family: Interferon
• UniProt Gene Name: IFNK
• UniProt Synonym Gene Names: UNQ6124/PRO20084; IFN-kappa
• UniProt Entry Name: IFNK_HUMAN

NCBI Description

This gene encodes a member of the type I interferon family. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. This protein is expressed in keratinocytes and the gene is found on chromosome 9, adjacent to the type I interferon cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

IFNK: May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon- stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin. Belongs to the alpha/beta interferon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: -

Cellular Component: extracellular space; extracellular region

Molecular Function: interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; cytokine and chemokine mediated signaling pathway; response to virus; regulation of MHC class I biosynthetic process; natural killer cell activation; humoral immune response; T cell activation during immune response; negative regulation of cell proliferation; response to exogenous dsRNA; regulation of transcription, DNA-dependent; B cell differentiation; positive regulation of innate immune response; natural killer cell activation during immune response; innate immune response; defense response to virus; positive regulation of peptidyl-serine phosphorylation of STAT protein