Human lymphocyte cytosolic protein 1 (L-plastin) ELISA Kit | LCP1 elisa kit

Human Plastin-2, LCP1 ELISA Kit

Cat. Num. AAA910940

• Gene Names: LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
• Reactivity: Human
• Synonyms: lymphocyte cytosolic protein 1 (L-plastin); Human Plastin-2; LCP1 ELISA Kit; Human Plastin-2 (LCP1) ELISA kit; CP64; DKFZp781A23186; FLJ25423; FLJ26114; FLJ39956; L-PLASTIN; LC64P; LPL; PLS2; L-plastin; L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P) ; Lymphocyte cytosolic protein-1 (plasmin) ; OTTHUMP; LCP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human LCP1. No significant cross-reactivity or interference between human LCP1 and analogues was observed.
• Sequence Length: 627

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCP1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LCP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 167614506
• NCBI GeneID: 3936
• NCBI Accession #: NP_002289.2
• NCBI GenBank Nucleotide #: NM_002298.4
• UniProt Accession #: P13796; Q5TBN4; B2R613
• Molecular Weight: 70,288 Da
• NCBI Official Full Name: plastin-2
• NCBI Official Synonym Full Names: lymphocyte cytosolic protein 1 (L-plastin)
• NCBI Official Symbol: LCP1
• NCBI Official Synonym Symbols: LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
• NCBI Protein Information: plastin-2; LCP-1; plastin 2; epididymis secretory protein Li 37; Lymphocyte cytosolic protein-1 (plasmin); bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)); L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P)
• UniProt Protein Name: Plastin-2
• Protein Family: Larval cuticle protein
• UniProt Gene Name: LCP1
• UniProt Synonym Gene Names: PLS2; LCP-1
• UniProt Entry Name: PLSL_HUMAN

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

L-plastin: Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69. Monomer. Interacts with AIF1. Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T- lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: ruffle; extracellular space; focal adhesion; cytoplasm; plasma membrane; actin filament bundle; stress fiber; actin filament; cytosol; filopodium; actin cytoskeleton; phagocytic cup

Molecular Function: actin filament binding; identical protein binding; calcium ion binding; actin binding

Biological Process: actin filament bundle formation; organ regeneration; cell migration; regulation of intracellular protein transport; T cell activation during immune response