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Testing Data

EZScreen Alanine Aminotransferase Assay Kit | ALT assay kit

EZScreen Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well)

Reactivity
Mammalian, Eukaryotes
Synonyms
EZScreen Alanine Aminotransferase; EZScreen Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well); ALT assay kit
Ordering
For Research Use Only!
Reactivity
Mammalian, Eukaryotes
Samples
Serum, plasmaandother bodily fluids
Cultured celllysates ofadherent or suspension cells, cell growth media
Tissue samples
Detection Method
Absorbance: 570nm or Fluorescence: Ex/Em 535/587nm
Features & Benefits
Simple procedure
Fast and convenient
Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.
Evaluation of pentose phosphate pathway
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months

Testing Data

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Testing Data
Related Product Information for ALT assay kit
Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: alpha-ketoglutarate + alanine ? glutamate + pyruvate It is commonly measured clinically as a part of a test liver function test to determine liver health. It is almost always measured in units/liter (U/L). In ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ?-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (lambdamax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.
Product Categories/Family for ALT assay kit

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Product Notes

The Mammalian, Eukaryotes ALT (Catalog #AAA849780) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849780 Assay Kit recognizes Mammalian, Eukaryotes ALT. It is sometimes possible for the material contained within the vial of "EZScreen Alanine Aminotransferase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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