Typical Testing Data/Standard Curve (for reference only)
Mammalian Indoleamine 2; 3-Dioxygenase 1 (IDO1) Assay Kit | IDO1 assay kit
Indoleamine 2; 3-Dioxygenase 1 (IDO1) Inhibitor Screening Kit
Reactivity
Mammalian
Synonyms
Indoleamine 2; 3-Dioxygenase 1 (IDO1); 3-Dioxygenase 1 (IDO1) Inhibitor Screening Kit; IDO1 assay kit
Reactivity
Mammalian
Sequence Length
489
Application Notes
Rapid; high-throughput screening of drugs and novel ligands.
Development of structure-activity relationship (SAR) models to predict IDO1 inhibition liability of novel compounds.
Development of structure-activity relationship (SAR) models to predict IDO1 inhibition liability of novel compounds.
Preparation and Storage
Store at -20 degree C for 12 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IDO1 assay kit
Background/Introduction: Indoleamine 2,3-Dioxygenase 1 (IDO1, EC 1.13.11.52) is a cytoplasmic hemoprotein that oxidizes tryptophan yielding N-formylkynurenine (NFK). In mammals, this reaction is the first and rate limiting step in the kynurenine catabolic pathway. IDO1 activity is low under normal physiological conditions, but is dramatically upregulated by proinflammatory cytokines such as interferon-gamma. This short-term activation of IDO1 occurs as part of the innate immune response and helps to inhibit the growth of pathogens and parasites. IDO1 activation can also promote host immune tolerance by exerting an immunosuppressive effect. IDO1 expression by tumor cells plays a substantial role in tumor immune tolerance, aiding tumors in evading detection and destruction. Chronic induction of IDO1 expression has been found in patients with many different types of cancer and increased IDO1 activity is correlated with more extensive disease spread and worsening prognosis. IDO1 has thus become an attractive pharmacological target for development of novel antineoplastics and adjuvants to increase the efficacy of conventional chemotherapy. The IDO1 Inhibitor Screening Kit enables rapid screening of test compounds for modulation of IDO1 activity. Unlike conventional colorimetric IDO1 assays based upon UV absorbance, the assay uses a fluorogenic developer that selectively reacts with NFK to produce a highly fluorescent product detectable in the visible range (Ex/Em = 402/488 nm). This ensures a high signal-to-background ratio and little interference due to short wavelength absorbance by test compounds. The assay is highly sensitive, has a simple no-wash protocol and is high-throughput adaptable. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.
Product Categories/Family for IDO1 assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
55,513 Da
NCBI Official Full Name
Indoleamine 2 3-dioxygenase 1
UniProt Protein Name
Indoleamine 2 3-dioxygenase 1
Protein Family
UniProt Gene Name
Ido1
Similar Products
Product Notes
The Mammalian IDO1 ido1 (Catalog #AAA846907) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA846907 Assay Kit recognizes Mammalian IDO1. Rapid; high-throughput screening of drugs and novel ligands. Development of structure-activity relationship (SAR) models to predict IDO1 inhibition liability of novel compounds. Researchers should empirically determine the suitability of the IDO1 ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Indoleamine 2; 3-Dioxygenase 1 (IDO1), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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