Human HDAC6 Assay Kit | HDAC6 assay kit

HDAC6 Inhibitor Screening Kit (Fluorometric)

Cat. Num. AAA846781

• Gene Names: HDAC6; HD6; JM21; CPBHM; PPP1R90
• Reactivity: Human
• Synonyms: HDAC6; HDAC6 Inhibitor Screening Kit (Fluorometric); HDAC6 assay kit

• Reactivity: Human
• Sequence Length: 1215

• Application Notes: Rapid; high-throughput screening of drugs and novel ligands. ; Development of structure-activity relationship (SAR) models to predict HDAC6 interaction liability of novel compounds.
• Preparation and Storage: Store at -80 degree C for 12 months.

Typical Standard Curve/Testing Data

Related Product Information for HDAC6 assay kit

Background/Introduction: Histone Deacetylase 6 (HDAC6, EC 3.5.1.98), is a class IIb HDAC enzyme that deacetylates an epsilon-N-acetyl lysine of histone and non-histone protein substrates. It is a unique member of HDAC family as it contains two deacetylase domains that are proposed to function independently of each other. HDAC6 can shuttle between the nucleus and cytoplasm, suggesting potential extra-nuclear functions by regulating the acetylation status of non-histone substrates. HDAC6 also affects transcription and translation by regulating the heat-shock protein 90 (Hsp90) and stress granules. Elevated HDAC6 activity is associated with cell motility and catalyzes increases alpha-tubulin deacetylation, thus influencing cancer cell metastasis. In addition, mutations in HDAC6 gene have been associated with Alzheimer's disease. HDAC6-selective inhibitors are considered as promising targets against autoimmune, inflammatory and oncology diseases such as breast and ovarian cancer. HDAC6 Inhibitor Screening Kit utilizes deacetylase activity of HDAC6 towards a synthetic acetylated-peptide substrate resulting in the release of an AFC fluorophore, which can be easily quantified using a conventional microplate reader. In the presence of an HDAC6-selective inhibitor (Tubacin), the deacetylase activity of HDAC6 is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HDAC6.

Product Categories/Family for HDAC6 assay kit

Epigenetics

NCBI and Uniprot Product Information

• NCBI GI #: 46623327
• NCBI GeneID: 10013
• Molecular Weight: 114,361 Da
• NCBI Official Full Name: HDAC6 protein
• NCBI Official Synonym Full Names: histone deacetylase 6
• NCBI Official Symbol: HDAC6
• NCBI Official Synonym Symbols: HD6; JM21; CPBHM; PPP1R90
• NCBI Protein Information: histone deacetylase 6
• UniProt Protein Name: Histone deacetylase 6
• UniProt Gene Name: HDAC6
• UniProt Synonym Gene Names: KIAA0901; HD6

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC6: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15. Belongs to the histone deacetylase family. HD type 2 subfamily.

Protein type: Deacetylase; EC 3.5.1.98; Nuclear receptor co-regulator; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: axon; caveola; cytoplasm; cytosol; dendrite; dynein complex; histone deacetylase complex; inclusion body; leading edge; microtubule; microtubule associated complex; multivesicular body; nucleoplasm; nucleus; perikaryon; perinuclear region of cytoplasm

Molecular Function: alpha-tubulin binding; beta-catenin binding; enzyme binding; histone deacetylase activity; histone deacetylase binding; Hsp90 protein binding; microtubule binding; misfolded protein binding; polyubiquitin binding; protein binding; tau protein binding; tubulin deacetylase activity; ubiquitin protein ligase binding

Biological Process: histone deacetylation; intracellular protein transport; lysosome localization; misfolded or incompletely synthesized protein catabolic process; negative regulation of oxidoreductase activity; negative regulation of protein complex disassembly; negative regulation of proteolysis; negative regulation of transcription, DNA-dependent; positive regulation of signal transduction; protein amino acid deacetylation; regulation of autophagy; regulation of gene expression, epigenetic; regulation of macroautophagy; regulation of protein stability; regulation of receptor activity; response to misfolded protein; response to organic substance; response to toxin

Disease: Chondrodysplasia With Platyspondyly, Distinctive Brachydactyly, Hydrocephaly, And Microphthalmia