IDO1 recombinant protein

Recombinant IDO1 protein

Cat. Num. AAA389090

• Gene Names: IDO1; IDO; INDO; IDO-1
• Synonyms: IDO1; Recombinant IDO1 protein; Indoleamine 2;3-dioxygenase 1; INDO; IDO-1; IDO1 recombinant protein

• Host: E Coli
• Form/Format: Recombinant IDO1 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol and 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Notes: This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Activity Assay Conditions: 400uM L-tryptophan was incubated with different concentrations of IDO1 protein in reaction buffer including 50mM potassium phosphate pH 6.5, 20mM ascorbic acid, 10uM methylene blue and 200ug/ml catalase for 1 hr at 37 degree C. The total 200ul reaction was stopped by the addition of 40ul 30% (w/v) trichloroacetic acid and heated at 65 degree C for 15 min. After cooled down to 4 degree C, the reaction was centrifuged at 1,125 g for 10 min. 100ul supernatant was transferred into 96-well plate and mixed with 100ul of 2% (w/v) p-DMAB in acetic acid. The yellow pigment derived from kynurenine was measured at 490nm.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant IDO1 protein gel 10% SDS-PAGE Coomassie staining MW: 47.5 kDa Purity: > 90%)

Testing Data

(Recombinant IDO1 protein activity assay 400 uM L-tryptophan was incubated with different concentrations of IDO1 protein in reaction buffer mixture. The reaction was stopped by the addition of 40 ul 30% (w/v) trichloroacetic acid and heated at 65 degree C for 15 min. After cooling down to 4 degree C, the reaction was centrifuged at 1,125 g for 10 min. 100 ul supernatant was transferred into 96-well plate and mixed with 100 ul of 2% (w/v) p-DMAB. The yellow pigment derived from kynurenine was measured at 490 nm.)

Related Product Information for IDO1 recombinant protein

Short Description: Recombinant IDO1 was expressed in E Coli as the full-length protein (accession number NP_002155.1) with an N-terminal 6xHis-tag. The molecular weight of the protein is 47.5 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: IDO1 (Indoleamine 2,3-dioxygenase 1, also known as IDO, INDO, or IDO-1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.

Product Categories/Family for IDO1 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4504577
• NCBI GeneID: 3620
• NCBI Accession #: NP_002155.1
• NCBI GenBank Nucleotide #: NM_002164.5
• UniProt Accession #: P14902; Q540B4; E5RGR8; F6M9T7
• Molecular Weight: 45,326 Da
• NCBI Official Full Name: indoleamine 2,3-dioxygenase 1
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 1
• NCBI Official Symbol: IDO1
• NCBI Official Synonym Symbols: IDO; INDO; IDO-1
• NCBI Protein Information: indoleamine 2,3-dioxygenase 1
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 1
• Protein Family: Indoleamine 2,3-dioxygenase
• UniProt Gene Name: IDO1
• UniProt Synonym Gene Names: IDO; INDO; IDO-1
• UniProt Entry Name: I23O1_HUMAN

NCBI Description

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Amino Acid Metabolism - tryptophan; Cell cycle regulation; EC 1.13.11.52; Oxidoreductase

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytosol

Molecular Function: electron carrier activity; metal ion binding; heme binding; indoleamine 2,3-dioxygenase activity; tryptophan 2,3-dioxygenase activity

Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; negative regulation of interleukin-10 production; positive regulation of chronic inflammatory response; tryptophan catabolic process to kynurenine; positive regulation of interleukin-12 production; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction