Transglutaminase 2 Active Protein | TGM2 active protein

Active Transglutaminase 2 (His-tagged), human recombinant

Cat. Num. AAA845198

• Gene Names: TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• Applications: SDS-Page
• Purity: >=90% by SDS-PAGE
• Synonyms: Transglutaminase 2; Active Transglutaminase 2 (His-tagged); human recombinant; TG2; tTG; tissue transglutaminase; transglutaminase C; TGC; protein-glutamine-gamma-glutamyltransferase; TGM2; TGM2 active protein

• Host: E Coli
• Purity/Purification: >=90% by SDS-PAGE
• Form/Format: Lyophilized from 5 mg/ml solution in a proprietary buffer. ; Appearance: Lyophilized powder
• Sequence Length: 687

• Applicable Applications for TGM2 active protein: SDS-PAGE; HPLC
• Application Notes: Recombinant human Transglutaminase can be used in inhibitor Screening Assays, Activity studies, Selectivity profiling, Western blotting, ELISA, and numerous similar applications.
• Solubility/Reconstitution Instructions: Reconstitute to 0.1-0.5 mU/ul in 20 mM Tris, 120 mM NaCl, 10 mM DTT, pH 7.5 containing 20% glycerol. Aliquot and store at -80 degree C. Avoid repeated freezing and thawing cycles.
• Activity (Specifications/test method): MyBioSource's Active His-tagged TG2 has been tested for its activity. It has a specific activity of >400 mU/mg. 1 U of TG2 activity is the amount of enzyme that will catalyze the formation of 1umol of hydroxamate per min as measured using Transglutaminase Activity Assay Kit (Colorimetric).
• Biological Activity: >400 mU/mg
• Results: >400 mU/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C

Testing Data

Related Product Information for TGM2 active protein

Background: Tissue transglutaminase, a 78-kDa calcium dependent enzyme (EC 2.3.2.13), is found both in the intracellular and the extracellular spaces of various types of tissues. TG2 crosslinks proteins between the epsilon-amino group of a lysine residue and the gamma-carboxamide group of glutamine residue, creating an inter- or intramolecular bond that is highly resistant to proteolysis (protein degradation). TG2 also possesses deamidation, GTP-binding/hydrolyzing, and isopeptidase activities. Intracellular TG2 is thought to play an important role in apoptosis, while extracellular TG2 has been linked to cell adhesion, ECM stabilization, wound healing, receptor signaling, cellular proliferation, and cellular motility.

Product Categories/Family for TGM2 active protein

Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesNeuroscience; ProteinsProteins and Enzymes; Other Proteins & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 39777597
• NCBI GeneID: 7052
• NCBI Accession #: NP_004604.2
• NCBI GenBank Nucleotide #: NM_004613.2
• UniProt Accession #: P21980; Q16436; Q6B838; Q9BTN7; Q9UH35; E1P5V9
• Molecular Weight: 79.5 kDa (1-687 aa + N-terminal polyhistidine tag).
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase 2 isoform a
• NCBI Official Synonym Full Names: transglutaminase 2
• NCBI Official Symbol: TGM2
• NCBI Official Synonym Symbols: TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase 2
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase 2
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM2
• UniProt Synonym Gene Names: TG(C); TGC; TGase C; TGase H; TGase-2
• UniProt Entry Name: TGM2_HUMAN

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of cell adhesion; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; apoptotic cell clearance; protein homooligomerization; positive regulation of inflammatory response; elevation of mitochondrial calcium ion concentration