Rabbit TGM2 Polyclonal Antibody | anti-TGM2 antibody

TGM2 antibody

Cat. Num. AAA9408764

• Gene Names: TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: TGM2; Polyclonal Antibody; TGM2 antibody; G-ALPHA-h; GNAH; TG2; TGC; anti-TGM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TGM2 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 687

• Applicable Applications for anti-TGM2 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human TGM2.
• Target Name: TGM2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TGM2 antibody.)

Related Product Information for anti-TGM2 antibody

Transglutaminase 2 (TGM2) is a calcium-dependent enzyme that cross-links both cytosolic and extracellular matrix proteins by catalyzing the formation of bonds between lysine and glutamine residues (1). This bifunctional enzyme also has intrinsic GTPase activity, and it has been suggested that regulation of the transamidase activity might be regulated through a G-protein coupled receptor-signaling pathway (2). In cross-linking peptides, TGM2 helps to regulate cytoskeletal structure, cell migration, apoptosis and cell-matrix adhesion. In addition, the enzyme plays an important role in wound healing and the immune response (3). TGM2 has exhibited kinase activity in vitro, with insulin-like growth factor-binding protein-3 (IGFBP-3) as one possible substrate (4). This widely expressed protein is localized to the cytosol and nucleus, but has also been isolated from the cell surface and extracellular matrix (reviewed in 5). Because of its interaction with a number of different substrates, and its role in the response to injury, TGM2 has been associated with the pathology of a number of human disorders. It has long been recognized as the major autoantigen in celiac disease (6); altered TGM2 expression or activity may be associated with Alzheimer disease, Huntington disease, arteriosclerosis, diabetes, and numerous forms of cancer (reviewed in 7).

Product Categories/Family for anti-TGM2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 39777597
• NCBI GeneID: 7052
• NCBI Accession #: NP_004604.2
• NCBI GenBank Nucleotide #: NM_004613.2
• UniProt Accession #: P21980; Q16436; Q6B838; Q9BTN7; Q9UH35; E1P5V9
• Molecular Weight: 38,671 Da
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase 2 isoform a
• NCBI Official Synonym Full Names: transglutaminase 2
• NCBI Official Symbol: TGM2
• NCBI Official Synonym Symbols: TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase 2
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase 2
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM2
• UniProt Synonym Gene Names: TG(C); TGC; TGase C; TGase H; TGase-2
• UniProt Entry Name: TGM2_HUMAN

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of cell adhesion; positive regulation of apoptosis; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; positive regulation of smooth muscle cell proliferation; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; apoptotic cell clearance; protein homooligomerization; elevation of mitochondrial calcium ion concentration; positive regulation of inflammatory response