Transglutaminase 2/TGM2 Recombinant Protein | TGM2 recombinant protein

Transglutaminase 2/TGM2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120683

• Gene Names: TGM2; TG2; TGC; GNAH; G-ALPHA-h
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Transglutaminase 2/TGM2; Transglutaminase 2/TGM2 Protein; Human; Recombinant (His Tag); Human TGM2/Transglutaminase 2 Protein (His Tag); G-ALPHA-h Protein; GNAH Protein; HEL-S-45 Protein; TG2 Protein; TGC Protein; transglutaminase 2; TGM2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, 2mM DTT, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala687

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human TGM2 (NP_004604.2)(Met 1-Ala 687) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TGM2 recombinant protein

Background: Protein-glutamine gamma-glutamyltransferase 2, also known as Tissue transglutaminase, Transglutaminase C, Transglutaminase-2, and TGM2, is a member of the transglutaminase superfamily. TGM2 plays a role in cell growth and survival through the anti-apoptosis signaling pathway. It is a calcium-dependent acyltransferase that also undergoes a GTP-binding/GTPase cycle even though it lacks any obvious sequence similarity with canonical GTP-binding (G) proteins. TGM2 is a multi-functional protein which catalyzes transamidation reactions or acts as a G-protein in intracellular signalling. As an enzyme which is responsible for the majority of transglutaminase (TG) activity in the brain, TGM2 is likely to play a modulatory role in nervous system development and has regulatory effect on neuronal cell death as well. Most importantly, numerous studies have presented data demonstrating that dysregulation of TGM2 may contribute to the pathogenesis of many neurodegenerative disorders, including Huntington's disease, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis as well as nervous system injuries.

References

Ruan Q, et al. (2007) Transglutaminase 2 in neurodegenerative disorders. Front Biosci. 12: 891-904.Ai L, et al. (2008) The transglutaminase 2 gene (TGM2), a potential molecular marker for chemotherapeutic drug sensitivity, is epigenetically silenced in breast cancer. Carcinogenesis. 29(3): 510-8.Filiano AJ, et al. (2010) Transglutaminase 2 protects against ischemic stroke. Neurobiol Dis. 39(3): 334-43.Park D, et al. (2010) Transglutaminase 2: a multi-functional protein in multiple subcellular compartments. Amino Acids. 39(3): 619-31.Miyoshi N, et al. (2010) TGM2 is a novel marker for prognosis and therapeutic target in colorectal cancer. Ann Surg Oncol. 17(4): 967-72.

NCBI and Uniprot Product Information

• NCBI GI #: 39777597
• NCBI GeneID: 7052
• NCBI Accession #: NP_004604.2
• NCBI GenBank Nucleotide #: NM_004613.2
• UniProt Accession #: P21980; Q16436; Q6B838; Q9BTN7; Q9UH35; E1P5V9
• Molecular Weight: 77,329 Da
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase 2 isoform a
• NCBI Official Synonym Full Names: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• NCBI Official Symbol: TGM2
• NCBI Official Synonym Symbols: TG2; TGC; GNAH; G-ALPHA-h
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase 2; TG(C); TGase C; TGase H; TGase-2; TGase-H; C polypeptide; transglutaminase C; transglutaminase H; transglutaminase-2; tissue transglutaminase; protein-glutamine-gamma-glutamyltransferase
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase 2
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM2
• UniProt Synonym Gene Names: TG(C); TGC; TGase C; TGase H; TGase-2
• UniProt Entry Name: TGM2_HUMAN

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: Transferase; EC 2.3.2.13

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of cell adhesion; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; positive regulation of apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of smooth muscle cell proliferation; blood vessel remodeling; protein homooligomerization; apoptotic cell clearance; elevation of mitochondrial calcium ion concentration; positive regulation of inflammatory response