Western Blot (WB)
(Western blot analysis of P2X4 expression in SW480 (A), THP1 (B) whole cell lysates.)
Rabbit anti-Human P2X4 Polyclonal Antibody | anti-P2RX4 antibody
Anti-P2X4 Antibody
Western Blot (WB)
(Western blot analysis of P2X4 expression in SW480 (A), THP1 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of P2X4 expression in SW480 (A), THP1 (B) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of P2X4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of P2X4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]
Uniprot Description
P2X4: Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. Belongs to the P2X receptor family.
Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 12q24.32
Cellular Component: cell junction; integral to nuclear inner membrane; integral to plasma membrane; lysosomal membrane; membrane; perinuclear region of cytoplasm; plasma membrane
Molecular Function: ATP binding; ATP-gated cation channel activity; cadherin binding; copper ion binding; protein binding; purinergic nucleotide receptor activity; receptor binding; zinc ion binding
Biological Process: blood coagulation; endothelial cell activation; membrane depolarization; positive regulation of calcium-mediated signaling; regulation of blood pressure; regulation of sodium ion transport; response to ATP; sensory perception of pain; signal transduction; transport
Research Articles on P2RX4
Similar Products
Product Notes
The P2RX4 p2rx4 (Catalog #AAA8247551) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-P2X4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's P2X4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: 1/500 - 1/2000, Immunofluorescence/Immunocytochemistry: 1/10 - 1/100. Researchers should empirically determine the suitability of the P2RX4 p2rx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P2X4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
