Testing Data
CD200/OX-2 Active Protein | CD200 active protein
Human CellExp CD200 / OX-2, human recombinant
Gene Names
CD200; MRC; MOX1; MOX2; OX-2
Applications
SDS-Page
Purity
>=98%
Synonyms
CD200/OX-2; Human CellExp CD200 / OX-2; human recombinant; CD200; MOX1; MOX2; MRC; OX-2; My033; CD200 active protein
Host
HEK293 cells
Purity/Purification
>=98%
Form/Format
Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Appearance: Lyophilized
Appearance: Lyophilized
Sequence Length
294
Applicable Applications for CD200 active protein
SDS-PAGE
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 mug/ml. The concentration of rhCD200 that produces 50% of the optimal binding response is approximately 0.5?16 ng/ml.
Results
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 mug/ml. The concentration of rhCD200 that produces 50% of the optimal binding response is approximately 0.5?16 ng/ml.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for CD200 active protein
Background: CD200 also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
Product Categories/Family for CD200 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 23.4 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 35-45 kDa due to glycosylation.
NCBI Official Full Name
OX-2 membrane glycoprotein isoform b
NCBI Official Synonym Full Names
CD200 molecule
NCBI Official Symbol
CD200
NCBI Official Synonym Symbols
MRC; MOX1; MOX2; OX-2
NCBI Protein Information
OX-2 membrane glycoprotein
UniProt Protein Name
OX-2 membrane glycoprotein
Protein Family
UniProt Gene Name
CD200
UniProt Synonym Gene Names
MOX1; MOX2
UniProt Entry Name
OX2G_HUMAN
NCBI Description
The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CD200: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13.2
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: protein binding
Biological Process: regulation of immune response
Research Articles on CD200
Similar Products
Product Notes
The CD200 cd200 (Catalog #AAA844737) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD200/OX-2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CD200 cd200 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD200/OX-2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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