Rabbit anti-Human LEF1 Polyclonal Antibody | anti-LEF1 antibody

LEF1 Antibody

Cat. Num. AAA9409519

• Gene Names: LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: LEF1; Polyclonal Antibody; LEF1 Antibody; LEF-1; TCF10; TCF7L3; TCF1ALPHA; anti-LEF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total LEF1 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 0.8 mg/ml (varies by lot)
• Sequence Length: 371

• Applicable Applications for anti-LEF1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:25-1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to residues near the C terminal of human lymphoid enhancer-binding factor 1
• Target Name: LEF1
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using at dilution 1/20.)

Related Product Information for anti-LEF1 antibody

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-LEF1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 195222732
• NCBI GeneID: 51176
• NCBI Accession #: NP_001124185.1
• NCBI GenBank Nucleotide #: NM_001130713.2
• UniProt Accession #: Q9UJU2; Q3ZCU4; Q9HAZ0; B4DG38; B7Z8E2; E9PDK3
• Molecular Weight: 34,103 Da
• NCBI Official Full Name: lymphoid enhancer-binding factor 1 isoform 2
• NCBI Official Synonym Full Names: lymphoid enhancer-binding factor 1
• NCBI Official Symbol: LEF1
• NCBI Official Synonym Symbols: LEF-1; TCF10; TCF7L3; TCF1ALPHA
• NCBI Protein Information: lymphoid enhancer-binding factor 1
• UniProt Protein Name: Lymphoid enhancer-binding factor 1
• Protein Family: Lymphoid enhancer-binding factor
• UniProt Gene Name: LEF1
• UniProt Synonym Gene Names: LEF-1; TCF1-alpha
• UniProt Entry Name: LEF1_HUMAN

NCBI Description

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

LEF-1: a transcription factor of the TCF/LEF family. Participates in the Wnt signaling pathway and regulates T-cell receptor alpha-C enhancer function. Activates transcription of target genes in the presence of CTNNB1 and EP300. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. Deletions in LEF1 have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Four human isoforms produced by alternative promoter usage and alternative splicing have been described. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4q23-q25

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; estrogen receptor activity; DNA binding; histone binding; sequence-specific DNA binding; gamma-catenin binding; caspase inhibitor activity; beta-catenin binding; estrogen receptor binding; chromatin binding; DNA bending activity; transcription factor binding; transcription factor activity

Biological Process: B cell proliferation; transcription from RNA polymerase II promoter; tongue development; radial glial cell differentiation in the forebrain; negative regulation of interleukin-4 production; hypothalamus development; paraxial mesoderm formation; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; T-helper 1 cell differentiation; alpha-beta T cell differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; forebrain neuroblast division; anatomical structure regression; forebrain neuron differentiation; BMP signaling pathway; negative regulation of interleukin-13 production; regulation of cell-cell adhesion; mammary gland development; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; muscle fiber development; neural crest cell migration; regulation of striated muscle development; embryonic limb morphogenesis; somitogenesis; dentate gyrus development; Wnt receptor signaling pathway; sensory perception of taste; neutrophil differentiation; negative regulation of striated muscle development; positive regulation of cell growth; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; patterning of blood vessels; osteoblast differentiation; formation of radial glial scaffolds; negative regulation of interleukin-5 production; epithelial to mesenchymal transition; T cell receptor V(D)J recombination; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-cell adhesion; sprouting angiogenesis; negative regulation of transcription, DNA-dependent; eye pigmentation; negative regulation of apoptosis; positive regulation of cell migration