SDS-PAGE
CD200 / OX-2 recombinant protein
Recombinant Human CD200 / OX-2 Protein (His & Fc tag)
Gene Names
CD200; MRC; MOX1; MOX2; OX-2
Purity
> 95 % as determined by SDS-PAGE
Synonyms
CD200 / OX-2; Recombinant Human CD200 / OX-2 Protein (His & Fc tag); CD200; MOX1; MOX2; MRC; OX-2; CD200 / OX-2 recombinant protein
Host
Human Cells
Purity/Purification
> 95 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH 7.4
Sequence Length
269
Application Notes
The recombinant human CD200/Fc is a disulfide-linked homodimer. The reduced monomer consists of 450 amino acids and has a predicted molecular mass of 50.5 kDa. As a result of glycosylation, the apparent molecular mass of rh CD200/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
Predicted N Terminal
Gln 31
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C
SDS-PAGE
SDS-PAGE
Related Product Information for CD200 / OX-2 recombinant protein
Background: CD200 (OX-2) is a cell surface glycoprotein that imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. Signals delivered through the CD200:CD200R axis have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. The role of CD200-CD200R signaling in immune regulation of the central nervous system has become a popular field of research in recent years. Many studies have shown that there is a close correlation between CD200-CD200R, microglia activation, and Parkinson's disease (PD). The ability of CD200 to suppress myeloid cell activation is critical for maintaining normal tissue homeostasis but may also enhance the survival of migratory neoplastic cells. CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 has been characterized as an important immunoregulatory molecule, increased expression of which can lead to decreased transplant rejection, autoimmunity, and allergic disease. Elevated CD200 expression has been reported to be associated with poor prognosis in a number of human malignancies. In addition, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
Description: A DNA sequence encoding the human CD200 (NP_005935.4) extracellular domain (Met 1-Gly 232) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Description: A DNA sequence encoding the human CD200 (NP_005935.4) extracellular domain (Met 1-Gly 232) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,975 Da
NCBI Official Full Name
OX-2 membrane glycoprotein isoform a
NCBI Official Synonym Full Names
CD200 molecule
NCBI Official Symbol
CD200
NCBI Official Synonym Symbols
MRC; MOX1; MOX2; OX-2
NCBI Protein Information
OX-2 membrane glycoprotein
UniProt Protein Name
OX-2 membrane glycoprotein
UniProt Gene Name
CD200
UniProt Synonym Gene Names
MOX1; MOX2
NCBI Description
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Uniprot Description
CD200: Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13.2
Cellular Component: cell-cell adherens junction; integral to plasma membrane; plasma membrane
Molecular Function: cell adhesion molecule binding; protein binding; protein homodimerization activity; receptor activity; receptor binding
Biological Process: cell recognition; heterophilic cell adhesion; homophilic cell adhesion; regulation of immune response
Research Articles on CD200 / OX-2
Similar Products
Product Notes
The CD200 / OX-2 cd200 (Catalog #AAA2546030) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human CD200/Fc is a disulfide-linked homodimer. The reduced monomer consists of 450 amino acids and has a predicted molecular mass of 50.5 kDa. As a result of glycosylation, the apparent molecular mass of rh CD200/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CD200 / OX-2 cd200 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD200 / OX-2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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