Typical Testing Data/Standard Curve (for reference only)
Myeloperoxidase (MPO) Assay Kit | MPO assay kit
Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
Screening MPO inhibitors for drug development
Cells: Adherent or suspension
Includes specific MPO Inhibitor [4-Aminobenzonic Hydrazide] to delineate MPO activity from total peroxidase activity in the sample.
Highly Sensitive assay for measuring MPO less than 2 muU per well
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Myeloperoxidase (MPO: EC 1.11.2.2) is a peroxidase abundantly expressed in neutrophil granulocytes. It catalyzes the hydrogen peroxide (H2O2) dependent oxidation of chloride anions (Cl-) to generate hypochlorite (HClO). During respiratory burst, neutrophils utilize MPO activity to exhibit cytotoxicity against tumor cells and pathogenic microorganisms. MPO also oxidizes various substances such as phenols and anilines, which can lead to inflammation. Increased MPO levels are associated with an increased risk of cardiovascular disease and myocardial infarction. Thus measurement of MPO peroxidation activity is crucial for disease prediction and therapeutics. MyBioSource's Myeloperoxidase (MPO) Peroxidation Activity Assay Kit, oxidizes a substrate to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to total peroxidase activity in the sample. A specific MPO inhibitor is provided, which suppresses peroxidase activity due to MPO. This permits differentiation of MPO-mediated peroxidation from the activity of other peroxidases which may be present in the samples. Our kit provides a sensitive, quick and easy method for detecting MPO activity. The assay is high-throughput adaptable and can detect less than 2 uU of MPO activity.
Summary: Detection method- Fluorescence (Ex/Em 535/587 nm)
Applications-
Measurement of MPO activity in various tissues/cells
Screening MPO inhibitors for drug development
NCBI and Uniprot Product Information
NCBI Description
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Uniprot Description
MPO: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Belongs to the peroxidase family. XPO subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.11.2.2; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q23.1
Cellular Component: extracellular space; azurophil granule; mitochondrion; lysosome; nucleus; secretory granule
Molecular Function: heparin binding; peroxidase activity; metal ion binding; heme binding; chromatin binding
Biological Process: respiratory burst during defense response; response to food; removal of superoxide radicals; hydrogen peroxide catabolic process; response to mechanical stimulus; defense response; response to lipopolysaccharide; response to yeast; response to oxidative stress; defense response to fungus; negative regulation of apoptosis; aging
Disease: Myeloperoxidase Deficiency; Alzheimer Disease
Research Articles on MPO
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Product Notes
The MPO mpo (Catalog #AAA841835) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of MPO activity in various tissues/cells Screening MPO inhibitors for drug development. Researchers should empirically determine the suitability of the MPO mpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myeloperoxidase (MPO), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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