Typical Testing Data/Standard Curve (for reference only)
Akt assay kit
Akt Activity Assay Kit, KinaseSTAR
Synonyms
Akt; Akt Activity Assay Kit; KinaseSTAR; Akt assay kit
Sequence Length
286
Samples
Cell and tissue lysates
Features & Benefits
Simple procedure
The kit is suitable for both research and drug discovery
The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
The kit is suitable for both research and drug discovery
The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Akt assay kit
Product Information: KinaseSTARTM Akt Activity Assay Kit: Akt Activity Assay Kit utilizing an Akt specific Antibody to Immunoprecipitate Akt from Cell lysate. Simple & Convenient. 40 Assays.
Background: Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3alpha as substrate. Phosphorylation of the GSK-3alpha can be analyzed by Western blot analysis using the phospho-GSK-3alpha specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Summary: Detection method- Western blot analysis using anti-Phospho-GSK-3alpha (Ser 21) antibody at 1:1000 dilutions
Species reactivity- Mammalian
Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3alpha as substrate.
Background: Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3alpha as substrate. Phosphorylation of the GSK-3alpha can be analyzed by Western blot analysis using the phospho-GSK-3alpha specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Summary: Detection method- Western blot analysis using anti-Phospho-GSK-3alpha (Ser 21) antibody at 1:1000 dilutions
Species reactivity- Mammalian
Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3alpha as substrate.
Product Categories/Family for Akt assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
32,995 Da
NCBI Official Full Name
Akt, partial
UniProt Protein Name
Akt
Protein Family
UniProt Gene Name
Akt
UniProt Entry Name
Q66XT6_ANOST
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Product Notes
The Akt akt (Catalog #AAA841832) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Akt, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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