Rat NGF beta ELISA Kit | NGF elisa kit

Rat NGF beta ELISA Kit

Cat. Num. AAA824760

• Gene Names: Ngf; Ngfb; beta-NGF
• Reactivity: Rat
• Applications: ELISA
• Synonyms: NGF beta; Rat NGF beta ELISA Kit; NGFB; Beta-nerve growth factor; Beta-NGF; NGF elisa kit

• Reactivity: Rat
• Specificity: The Rat IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-6 proteins within the range of 62.5 pg/ml-4000 pg/ml.
• Sequence Length: 241

• Applicable Applications for NGF elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
• Sensitivity: 30 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NGF elisa kit

Principle of the Assay: The MyBioSource's Rat IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-6 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Rat IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Interleukin 6 is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis. IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction. The rat, mouse and human IL-6 cDNAs have been cloned. Rat IL-6 cDNA encodes a 211 amino acid residue precursor polypeptide with a hydrophobic signal peptide that is cleaved to generate the 187 aa residue mature protein. At the protein sequence level, there is approximately 39% identity between rat and human, and 87% identity between mouse and rat IL-6. IL-6 is expressed by a variety of normal and transformed lymphoid and non-lymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections,trauma, autoimmune diseases, inflammations and malignancies.

NCBI and Uniprot Product Information

• NCBI GI #: 456367246
• NCBI GeneID: 310738
• NCBI Accession #: NP_001263984.1
• NCBI GenBank Nucleotide #: NM_001277055.1
• UniProt Accession #: P25427
• Molecular Weight: 27,010 Da
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb; beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF
• UniProt Entry Name: NGF_RAT

Uniprot Description

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.