Rat Nerve Growth Factor (NGF) ELISA Kit | NGF elisa kit

Rat Nerve Growth Factor (NGF) ELISA Kit

Cat. Num. AAA453168

• Gene Names: Ngf; Ngfb; beta-NGF
• Reactivity: Rat
• Synonyms: Nerve Growth Factor (NGF); Rat Nerve Growth Factor (NGF) ELISA Kit; NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor; NGF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of NGF. No significant cross-reactivity or interference between NGF and analogues was observed.
• Sequence Length: 241

• Samples: Bovine Serum, Plasma, Tissue Homogenates, Cell Lysates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 125-8000pg/mL
• Sensitivity: 30pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NGF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NGF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NGF elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NGF in bovine serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 456367246
• NCBI GeneID: 310738
• NCBI Accession #: NP_001263984.1
• NCBI GenBank Nucleotide #: NM_001277055.1
• UniProt Accession #: P25427
• Molecular Weight: 27,010 Da
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor
• NCBI Official Symbol: Ngf
• NCBI Official Synonym Symbols: Ngfb; beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: Ngf
• UniProt Synonym Gene Names: Ngfb; Beta-NGF

Uniprot Description

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.