Typical Testing Data/Standard Curve
Mouse ADAM12 ELISA Kit | ADAM12 elisa kit
Mouse ADAM12 ELISA Kit
Gene Names
Adam12; Mltna; mKIAA4001
Reactivity
Mouse
Applications
ELISA
Synonyms
ADAM12; Mouse ADAM12 ELISA Kit; MLTN; Disintegrin and metalloproteinase domain-containing protein 12; ADAM 12; Meltrin-alpha; ADAM12 elisa kit
Reactivity
Mouse
Specificity
The Mouse ADAM12 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse ADAM12 proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
903
Applicable Applications for ADAM12 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for ADAM12 elisa kit
Principle of the Assay||The Mouse ADAM12 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse ADAM12 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse ADAM12 coated on a 96-well plate. Standards and samples are pipetted into the wells and ADAM12 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse ADAM12 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ADAM12 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
Background/Introduction: ADAM12 (Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
98,504 Da
NCBI Official Full Name
Disintegrin and metalloproteinase domain-containing protein 12
NCBI Official Synonym Full Names
a disintegrin and metallopeptidase domain 12 (meltrin alpha)
NCBI Official Symbol
Adam12
NCBI Official Synonym Symbols
Mltna; mKIAA4001
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 12
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 12
Protein Family
UniProt Gene Name
Adam12
UniProt Synonym Gene Names
Mltna; ADAM 12
UniProt Entry Name
ADA12_MOUSE
Uniprot Description
ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.
Protein type: EC 3.4.24.-; Protease; Motility/polarity/chemotaxis; Membrane protein, integral
Cellular Component: integral to membrane; membrane; mitochondrion; nucleoplasm; plasma membrane
Molecular Function: hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; protein binding; SH3 domain binding; zinc ion binding
Biological Process: cell adhesion; proteolysis
Research Articles on ADAM12
Similar Products
Product Notes
The Mouse ADAM12 adam12 (Catalog #AAA8244723) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244723 ELISA Kit recognizes Mouse ADAM12. AAA Biotech's ADAM12 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the ADAM12 adam12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAM12, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
