Mouse Lumican ELISA Kit | LUM elisa kit

Mouse Lumican ELISA Kit

Cat. Num. AAA824730

• Gene Names: Lum; Ldc; SLRR2D
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Lumican; Mouse Lumican ELISA Kit; LDC; SLRR2D; Keratan sulfate proteoglycan lumican; KSPG lumican; LUM elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Lumican ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Lumican proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 338

• Applicable Applications for LUM elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for LUM elisa kit

Principle of the Assay||The Mouse Lumican ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Lumican in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse Lumican coated on a 96-well plate. Standards and samples are pipetted into the wells and Lumican present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Lumican antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lumican bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

NCBI and Uniprot Product Information

• NCBI GI #: 160333372
• NCBI GeneID: 17022
• NCBI Accession #: NP_032550.2
• NCBI GenBank Nucleotide #: NM_008524.2
• UniProt Accession #: P51885; Q3TP25; Q99JZ3; Q9CXK0
• Molecular Weight: 38,265 Da
• NCBI Official Full Name: lumican
• NCBI Official Synonym Full Names: lumican
• NCBI Official Symbol: Lum
• NCBI Official Synonym Symbols: Ldc; SLRR2D
• NCBI Protein Information: lumican; KSPG lumican; keratan sulfate proteoglycan lumican
• UniProt Protein Name: Lumican
• Protein Family: Lumican
• UniProt Gene Name: Lum
• UniProt Synonym Gene Names: Lcn; Ldc; KSPG lumican
• UniProt Entry Name: LUM_MOUSE

Uniprot Description

LUM: a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; fibrillar collagen; extracellular region

Molecular Function: collagen binding

Biological Process: collagen fibril organization; visual perception; positive regulation of transcription from RNA polymerase II promoter