Typical Testing Data/Standard Curve (for reference only)
(Rat ADAM12 PicoKine ELISA Kit standard curve )
Rat ADAM12 ELISA Kit | ADAM12 elisa kit
Rat ADAM12 PicoKine ELISA Kit
Reactivity
Rat
Synonyms
ADAM12; Rat ADAM12 PicoKine ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 12; ADAM metallopeptidase domain 12; ADA12_HUMAN; ADAM 12; MCMP; MCMPMltna; Meltrin-alpha; MLTN; MLTNA; ADAM12 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat ADAM12
Sequence Length
903
Samples
Cell culture supernates, serum and plasma (heparin)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat ADAM12 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat ADAM12 PicoKine ELISA Kit standard curve )
Related Product Information for ADAM12 elisa kit
Principle of the assay: rat ADAM12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM12 has been precoated onto 96-well plates. Standards(CHO, R29-S513) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat ADAM12 amount of sample captured in plate.
Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
98,663 Da
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 12
NCBI Official Symbol
Adam12
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 12; ADAM metallopeptidase domain 12; meltrin alpha; a disintegrin and metallopeptidase domain 12 (meltrin alpha)
UniProt Protein Name
Protein Adam12
Protein Family
UniProt Gene Name
Adam12
UniProt Entry Name
F1M9Z9_RAT
Uniprot Description
ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.
Protein type: EC 3.4.24.-; Protease; Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm; mitochondrion; plasma membrane
Molecular Function: zinc ion binding; metalloendopeptidase activity; SH3 domain binding
Biological Process: proteolysis
Research Articles on ADAM12
Similar Products
Product Notes
The Rat ADAM12 adam12 (Catalog #AAA177157) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177157 ELISA Kit recognizes Rat ADAM12. It is sometimes possible for the material contained within the vial of "ADAM12, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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