CASP8 sirna

CASP8 siRNA (Mouse)

Cat. Num. AAA8241403

• Gene Names: Casp8; MACH; Mch5; FLICE; CASP-8
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP8; CASP8 siRNA (Mouse); Caspase-8; CASP-8; CASP8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CASP8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 480

• Applicable Applications for CASP8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CASP8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP8 sirna

siRNA to inhibit CASP8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 122056578
• NCBI GeneID: 12370
• NCBI Accession #: NP_001073595.1
• NCBI GenBank Nucleotide #: NM_001080126.1
• UniProt Accession #: O89110; O35669
• Molecular Weight: 55,357 Da
• NCBI Official Full Name: caspase-8 isoform 1
• NCBI Official Synonym Full Names: caspase 8
• NCBI Official Symbol: Casp8
• NCBI Official Synonym Symbols: MACH; Mch5; FLICE; CASP-8
• NCBI Protein Information: caspase-8
• UniProt Protein Name: Caspase-8
• Protein Family: Caspase
• UniProt Gene Name: Casp8
• UniProt Synonym Gene Names: CASP-8
• UniProt Entry Name: CASP8_MOUSE

NCBI Description

This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, an initiator of apoptotic cell death, is activated by death-inducing tumor necrosis family receptors and targets downstream effectors. In mouse deficiency of this gene can cause embryonic lethality. This protein may have a role in embryogenesis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]

Uniprot Description

CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Interacts with human cytomegalovirus/HHV-5 protein vICA/UL36; this interaction inhibits CASP8 activation. Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. Belongs to the peptidase C14A family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.61; Protease; Apoptosis

Cellular Component: nucleoplasm; neuron projection; protein complex; mitochondrion; Noc1p-Noc2p complex; cytoplasm; CD95 death-inducing signaling complex; microtubule organizing center; nucleus; cytosol; lipid raft

Molecular Function: peptidase activity; protein binding; hydrolase activity; protein heterodimerization activity; ubiquitin protein ligase binding; cysteine-type endopeptidase activity; endopeptidase activity; death receptor binding; protein complex binding; tumor necrosis factor receptor binding; cysteine-type peptidase activity

Biological Process: caspase activation; cardiac muscle development; macrophage differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of proteolysis; apoptosis; protein heterooligomerization; heart development; negative regulation of I-kappaB kinase/NF-kappaB cascade; proteolysis; regulation of apoptosis; response to ethanol; induction of apoptosis via death domain receptors; proteolysis involved in cellular protein catabolic process; angiogenesis; positive regulation of macrophage differentiation; neural tube formation