Western Blot (WB)
(Western blot analysis of anti-LATS1 Antibody (N-term) in K562 cell line lysates (35ug/lane). LATS1 (arrow) was detected using the purified Pab.)
Rabbit LATS1 Polyclonal Antibody | anti-LATS1 antibody
LATS1 Antibody (N-term)
IHC-P~~1:50~~100
Western Blot (WB)
(Western blot analysis of anti-LATS1 Antibody (N-term) in K562 cell line lysates (35ug/lane). LATS1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-LATS1 Antibody (N-term) in K562 cell line lysates (35ug/lane). LATS1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Yang, X., et al., Nat. Cell Biol. 6(7):609-617 (2004).
Kamikubo, Y., et al., J. Biol. Chem. 278(20):17609-17614 (2003).
Hisaoka, M., et al., Lab. Invest. 82(10):1427-1435 (2002).
Hirota, T., et al., J. Cell Biol. 149(5):1073-1086 (2000).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. Two protein-coding transcripts and one non-protein coding transcript have been found for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
LATS1: an AGC kinase of the NDR family of kinases. Localized to the mitotic apparatus and specifically phosphorylated during mitosis. A likely tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDC2 kinase activity, causing G2 arrest. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. Complexes with CDC2 in early mitosis. LATS1-associated CDC2 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated zyxin, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Knockout mice are susceptible to soft-tissue sarcomas and sensitive to chemical carcinogenesis. Human soft tissue sarcomas have downregulated, mutated, and/or hypermethylated LATS1. Transgenic expression blocks anchorage independent growth in culture and tumor growth in xenografts. Two alternatively spliced isoforms of the human proteinhave been described.
Protein type: Kinase, protein; Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; AGC group; NDR family
Chromosomal Location of Human Ortholog: 6q25.1
Cellular Component: spindle pole; microtubule organizing center; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; protein kinase binding; ATP binding
Biological Process: keratinocyte differentiation; mitosis; regulation of actin filament polymerization; hormone-mediated signaling; cell division; sister chromatid segregation; cytoplasmic sequestering of protein; negative regulation of cyclin-dependent protein kinase activity; regulation of protein complex assembly; G2/M transition of mitotic cell cycle; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation
Research Articles on LATS1
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Product Notes
The LATS1 lats1 (Catalog #AAA9203165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The LATS1 Antibody (N-term) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's LATS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000 IHC-P~~1:50~~100. Researchers should empirically determine the suitability of the LATS1 lats1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LATS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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