CASP4 sirna

CASP4 siRNA (Mouse)

Cat. Num. AAA8238753

• Gene Names: Casp4; Caspl; ich-3; CASP-4; Casp11; CASP-11
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP4; CASP4 siRNA (Mouse); CASP11; CASPL; ICH3; Caspase-4; CASP-4; Caspase-11; CASP-11; Protease ICH-3; CASP4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CASP4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 373

• Applicable Applications for CASP4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CASP4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP4 sirna

siRNA to inhibit CASP4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157951738
• NCBI GeneID: 12363
• NCBI Accession #: NP_031635.2
• NCBI GenBank Nucleotide #: NM_007609.3
• UniProt Accession #: P70343; O08735; Q3TAF3
• Molecular Weight: 42,742 Da
• NCBI Official Full Name: caspase-4
• NCBI Official Synonym Full Names: caspase 4, apoptosis-related cysteine peptidase
• NCBI Official Symbol: Casp4
• NCBI Official Synonym Symbols: Caspl; ich-3; CASP-4; Casp11; CASP-11
• NCBI Protein Information: caspase-4
• UniProt Protein Name: Caspase-4
• Protein Family: Caspase
• UniProt Gene Name: Casp4
• UniProt Synonym Gene Names: Casp11; Caspl; Ich3; CASP-4; CASP-11
• UniProt Entry Name: CASP4_MOUSE

NCBI Description

This gene encodes a member of the cysteine proteases that plays important roles in apoptosis, cell migration and the inflammatory response. The encoded protein mediates production of pro-inflammatory cytokines by macrophages upon bacterial infection. Mice lacking the encoded protein are resistant to endotoxic shock induced by lipopolysaccharide. A 5-bp deletion encompassing a splice acceptor junction resulting in alternate splicing and a shorter non-functional isoform in certain mouse strains has been described. Although its official nomenclature is "caspase 4, apoptosis-related cysteine peptidase", this gene and its encoded protein have historically been called caspase 11. This gene is present in a cluster of three caspase genes on chromosome 9. [provided by RefSeq, Apr 2015]

Uniprot Description

CASP4: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.64; Protease; Apoptosis

Cellular Component: neuron projection; protein complex; membrane; mitochondrion; cell soma; endoplasmic reticulum; cytoplasm

Molecular Function: peptidase activity; hydrolase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; apoptosis; regulation of inflammatory response; germ cell programmed cell death; actin filament organization; positive regulation of interleukin-1 beta secretion; proteolysis