TRPC4 sirna

TRPC4 siRNA (Human)

Cat. Num. AAA8238442

• Gene Names: TRPC4; TRP4; HTRP4; HTRP-4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRPC4; TRPC4 siRNA (Human); Short transient receptor potential channel 4; TrpC4; Trp-related protein 4; hTrp-4; hTrp4; TRPC4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRPC4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 893

• Applicable Applications for TRPC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRPC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRPC4 sirna

siRNA to inhibit TRPC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209863026
• NCBI GeneID: 7223
• NCBI Accession #: NP_001129427.1
• NCBI GenBank Nucleotide #: NM_001135955.1
• UniProt Accession #: Q9UBN4; Q15721; Q3SWS6; Q96P03; Q96P04; Q96P05; Q9UIB0; Q9UIB1; Q9UIB2; B1ALE0; B1ALE1; B1ALE2
• Molecular Weight: 36,759 Da
• NCBI Official Full Name: short transient receptor potential channel 4 isoform beta
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily C, member 4
• NCBI Official Symbol: TRPC4
• NCBI Official Synonym Symbols: TRP4; HTRP4; HTRP-4
• NCBI Protein Information: short transient receptor potential channel 4
• UniProt Protein Name: Short transient receptor potential channel 4
• Protein Family: Short transient receptor potential channel
• UniProt Gene Name: TRPC4
• UniProt Synonym Gene Names: TrpC4; hTrp-4; hTrp4
• UniProt Entry Name: TRPC4_HUMAN

NCBI Description

This gene encodes a member of the canonical subfamily of transient receptor potential cation channels. The encoded protein forms a non-selective calcium-permeable cation channel that is activated by Gq-coupled receptors and tyrosine kinases, and plays a role in multiple processes including endothelial permeability, vasodilation, neurotransmitter release and cell proliferation. Single nucleotide polymorphisms in this gene may be associated with generalized epilepsy with photosensitivity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

TRPC4: Form a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion. Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, calcium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: cortical cytoskeleton; cell surface; basolateral plasma membrane; plasma membrane; intercellular junction; caveola

Molecular Function: protein binding; store-operated calcium channel activity; cadherin binding; beta-catenin binding

Biological Process: axon guidance; calcium ion transport; gamma-aminobutyric acid secretion; oligodendrocyte differentiation; transmembrane transport