Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse ENG(CD105) expression on bEnd.3 cells. Cells were stained with purified anti-Mouse ENG(CD105), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Mouse Endoglin/CD105 Monoclonal Antibody | anti-ENG antibody
Recombinant Anti-Endoglin/CD105 Antibody, Rabbit Monoclonal
No cross-reactivity in ELISA with Human CD105
ELISA: 1:25,000-1:50,000
FC/FACS/FCM: 1:100-1:500
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse ENG(CD105) expression on bEnd.3 cells. Cells were stained with purified anti-Mouse ENG(CD105), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse ENG(CD105) expression on bEnd.3 cells. Cells were stained with purified anti-Mouse ENG(CD105), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Western Blot (WB)
(Anti-ENG rabbit monoclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:45 kDa)
Western Blot (WB)
(Anti-ENG rabbit monoclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:45 kDa)
NCBI and Uniprot Product Information
Uniprot Description
ENG: Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Interacts with TCTEX1D4. Interacts with ARRB2. Endoglin is restricted to endothelial cells in all tissues except bone marrow. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm; extracellular space; cell surface; focal adhesion; membrane; cytoplasm; integral to membrane; receptor complex; external side of plasma membrane
Molecular Function: transforming growth factor beta receptor activity; protein binding; glycosaminoglycan binding; protein homodimerization activity; transforming growth factor beta binding; galactose binding; punt binding; transforming growth factor beta receptor, cytoplasmic mediator activity
Biological Process: wound healing; heart development; positive regulation of collagen biosynthetic process; cell motility involved in cell locomotion; positive regulation of systemic arterial blood pressure; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; smooth muscle development; extracellular matrix disassembly; regulation of transforming growth factor beta receptor signaling pathway; central nervous system vasculogenesis; venous blood vessel morphogenesis; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway; negative regulation of protein amino acid autophosphorylation; heart looping; angiogenesis; vasculogenesis; cell adhesion; negative regulation of cell migration; positive regulation of BMP signaling pathway; cell migration; negative regulation of nitric-oxide synthase activity; patterning of blood vessels; positive regulation of angiogenesis; negative regulation of endothelial cell proliferation; response to hypoxia; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation
Research Articles on ENG
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Product Notes
The ENG eng (Catalog #AAA8111509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Endoglin/CD105 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Endoglin/CD105 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS/FCM). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 FC/FACS/FCM: 1:100-1:500. Researchers should empirically determine the suitability of the ENG eng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endoglin/CD105, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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