BCL2 sirna

BCL2 siRNA (Rat)

Cat. Num. AAA8236867

• Gene Names: Bcl2; Bcl-2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BCL2; BCL2 siRNA (Rat); BCL-2; Apoptosis regulator Bcl-2; BCL2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: BCL2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 236

• Applicable Applications for BCL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat BCL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BCL2 sirna

siRNA to inhibit BCL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 8392974
• NCBI GeneID: 24224
• NCBI Accession #: NP_058689.1
• NCBI GenBank Nucleotide #: NM_016993.1
• UniProt Accession #: P49950; Q62837; Q64032
• Molecular Weight: 26,622 Da
• NCBI Official Full Name: apoptosis regulator Bcl-2
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 2
• NCBI Official Symbol: Bcl2
• NCBI Official Synonym Symbols: Bcl-2
• NCBI Protein Information: apoptosis regulator Bcl-2
• UniProt Protein Name: Apoptosis regulator Bcl-2
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: Bcl2
• UniProt Synonym Gene Names: Bcl-2
• UniProt Entry Name: BCL2_RAT

NCBI Description

an anti-apoptotic protein; involved in inhibiting cell death in many different cell types [RGD, Feb 2006]

Uniprot Description

Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.

Protein type: Apoptosis; Membrane protein, integral; Oncoprotein; Autophagy

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; mitochondrion; endoplasmic reticulum; cell; integral to membrane; cytosol; pore complex; mitochondrial outer membrane; membrane; mitochondrial membrane; cytoplasm; intracellular; nucleus; myelin sheath

Molecular Function: identical protein binding; BH domain binding; protein homodimerization activity; protease binding; protein phosphatase 2A binding; channel activity; channel inhibitor activity; protein phosphatase binding; transcription factor binding; protein binding; sequence-specific DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; BH3 domain binding

Biological Process: positive regulation of catalytic activity; focal adhesion formation; developmental growth; pigment granule organization and biogenesis; regulation of cell cycle; response to toxin; ear development; negative regulation of myeloid cell apoptosis; B cell receptor signaling pathway; regulation of protein localization; negative regulation of ossification; oligodendrocyte differentiation; regulation of transmembrane transporter activity; negative regulation of neuron apoptosis; kidney development; T cell differentiation; response to drug; spleen development; positive regulation of neuron maturation; axon regeneration; cell aging; regulation of calcium ion transport; positive regulation of cell growth; response to ethanol; DNA damage response, signal transduction resulting in induction of apoptosis; response to estrogen stimulus; regulation of gene expression; response to heat; negative regulation of osteoblast proliferation; response to oxidative stress; lymphocyte homeostasis; negative regulation of apoptosis; positive regulation of pigmentation; positive regulation of smooth muscle cell migration; response to L-ascorbic acid; response to insulin stimulus; response to organic substance; negative regulation of cell proliferation; positive regulation of skeletal muscle fiber development; ureteric bud development; positive regulation of cell proliferation; regulation of viral genome replication; transmembrane transport; negative regulation of cell migration; response to nutrient; leukocyte homeostasis; aging; mesenchymal cell development; response to aluminum ion; CD8-positive, alpha-beta T cell lineage commitment; response to inorganic substance; B cell lineage commitment; positive regulation of peptidyl-serine phosphorylation; response to UV-B; cell proliferation; peptidyl-serine phosphorylation; endoplasmic reticulum calcium ion homeostasis; pigmentation; response to copper ion; ureteric bud branching; homeostasis of number of cells within a tissue; response to hypoxia; brain development; negative regulation of cell growth; response to nicotine; renal system process; protein polyubiquitination; response to glucocorticoid stimulus; T cell differentiation in the thymus; lymphoid progenitor cell differentiation; positive regulation of multicellular organism growth; glomerulus development; response to organic cyclic substance; post-embryonic development; response to caffeine; regulation of apoptosis; cochlear nucleus development; cellular response to glucose starvation; cell-cell adhesion; regulation of mitochondrial membrane potential; response to folic acid; positive regulation of B cell proliferation; negative regulation of mitotic cell cycle; T cell homeostasis; response to electrical stimulus; cell growth; defense response to virus; response to corticosterone stimulus; release of cytochrome c from mitochondria; regulation of protein homodimerization activity; actin filament organization; digestive tract morphogenesis; organ growth; organ morphogenesis; induction of apoptosis via death domain receptors; response to steroid hormone stimulus; B cell differentiation; regulation of pigmentation during development; gland morphogenesis; regulation of mitochondrial membrane permeability; regulation of nitrogen utilization; metanephros development; oocyte development; B cell proliferation; response to peptide hormone stimulus; regulation of protein heterodimerization activity; response to cAMP; behavioral fear response; melanin metabolic process; apoptosis; regulation of cell-matrix adhesion; negative regulation of retinal cell programmed cell death; pigmentation during development; cell morphogenesis; regulation of protein stability; protein amino acid dephosphorylation; apoptotic mitochondrial changes; ovarian follicle development; B cell homeostasis; regulation of catalytic activity; positive regulation of melanocyte differentiation; melanocyte differentiation; response to gamma radiation; hemopoiesis; response to iron ion; negative regulation of cellular pH reduction; ossification; hair follicle morphogenesis; thymus development; male gonad development; peptidyl-threonine phosphorylation; regulation of programmed cell death; cellular calcium ion homeostasis; immune system development; neuron apoptosis; T cell lineage commitment; response to hydrogen peroxide; axonogenesis; response to cytokine stimulus; response to DNA damage stimulus; induction of apoptosis by oxidative stress; growth