Mouse anti-Human Annexin A2 Monoclonal Antibody | anti-A2 antibody

Annexin A2 Antibody

Cat. Num. AAA474162

• Gene Names: Anxa2; ANX2
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation
• Synonyms: Annexin A2; Monoclonal Antibody; Annexin A2 Antibody; Annexin A2 M009; anti-A2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M009
• Specificity: Clone M009 detects a 36kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, HUVEC, and PC3 cells. The antibody also works for ELISA, immunocytochemical labeling, and immunoprecipitation applications. In addition, mass spectrometry analysis of immunoprecipitates using AM0091 in human A431 cell lysates confirms that this antibody only detects Annexin A2 protein.
• Form/Format: Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-A2 antibody: Western Blot (WB), Immunocytochemistry (ICC), ELISA (EIA), Immunoprecipitation-Mass Spectrometry (IP-MS)
• Application Notes: ELISA: 1:1000; ICC: 1:100; IP-MS: 1:100; WB: 1:1000
• Immunogen: Clone M009 was generated from a proprietary antigen related to human Annexin A2 in MDA-MB-231 breast cancer cell line.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human A431 (lane 1), MDA-MB-231 (lane 2), LNCaP (lane 3), MeWo (lane 4), HUVEC (lane 5), Jurkat (lane 6), K562 (lane 7), and PC3 (lane 8) cell lysates. The blot was probed with mouse monoclonal anti-Annexin A2 antibody (AM0091) at 1:500 (lanes 1-8).)

Related Product Information for anti-A2 antibody

The Annexin family of is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.

References

Grewal, T. & Enrich, C. (2006) Bioessays 28(12):1211.
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.

NCBI and Uniprot Product Information

• NCBI GI #: 9845234
• NCBI GeneID: 56611
• NCBI Accession #: NP_063970.1
• NCBI GenBank Nucleotide #: NM_019905.1
• UniProt Accession #: Q07936
• Molecular Weight: 38,893 Da
• NCBI Official Full Name: annexin A2
• NCBI Official Synonym Full Names: annexin A2
• NCBI Official Symbol: Anxa2
• NCBI Official Synonym Symbols: ANX2
• NCBI Protein Information: annexin A2
• UniProt Protein Name: Annexin A2
• Protein Family: Annexin
• UniProt Gene Name: Anxa2
• UniProt Synonym Gene Names: Anx2; PAP-IV

NCBI Description

a plasminogen receptor; may be involved in cellular transformation [RGD, Feb 2006]

Uniprot Description

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.