NPFF sirna

NPFF siRNA (Human)

Cat. Num. AAA8228001

• Gene Names: NPFF; FMRFAL
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NPFF; NPFF siRNA (Human); Pro-FMRFamide-related neuropeptide FF; FMRFamide-related peptides; NPFF sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NPFF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 113

• Applicable Applications for NPFF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NPFF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NPFF sirna

siRNA to inhibit NPFF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503769
• NCBI GeneID: 8620
• NCBI Accession #: NP_003708.1
• NCBI GenBank Nucleotide #: NM_003717.2
• UniProt Accession #: O15130; Q3SXL4
• Molecular Weight: 12,991 Da
• NCBI Official Full Name: pro-FMRFamide-related neuropeptide FF preproprotein
• NCBI Official Synonym Full Names: neuropeptide FF-amide peptide precursor
• NCBI Official Symbol: NPFF
• NCBI Official Synonym Symbols: FMRFAL
• NCBI Protein Information: pro-FMRFamide-related neuropeptide FF
• UniProt Protein Name: Pro-FMRFamide-related neuropeptide FF
• Protein Family: Pro-FMRFamide-related neuropeptide
• UniProt Gene Name: NPFF
• UniProt Synonym Gene Names: NPSF; NPFF; NPAF
• UniProt Entry Name: NPFF_HUMAN

NCBI Description

FMFRamide-related protein precursor plays a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. FMRFAL encodes a preproprotein which is cleaved to form two active peptides with similar function. [provided by RefSeq, Jul 2008]

Uniprot Description

NPFF: Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels. Belongs to the FARP (FMRFamide related peptide) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular space; dendrite; extracellular region; perikaryon; nerve terminal; vesicle

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding; receptor binding

Biological Process: response to drug; negative regulation of heart rate; maternal process involved in pregnancy; response to morphine; negative regulation of appetite; negative regulation of insulin secretion; vasopressin secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; spinal cord development; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; positive regulation of blood pressure; acute inflammatory response to antigenic stimulus