Western Blot (WB)
((0.5ug/ml) staining of mouse fibroblast lysates transiently expressing different siRNA in second and third lane (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat HYPB/SETD2 Polyclonal Antibody | anti-SETD2 antibody
Goat anti-HYPB/SETD2 (internal region) Antibody
Western Blot: Approx 290kDa band observed in lysates of mouse fibroblasts. The observed band is knocked down by two different siRNA molecules. Calculated MW of 231kDa according to NP_054878.3). Data obtained from anonymous customer and obtained from a previous batch (from a different goat). The Ponceau red demonstrates proper protein loading. Recommended concentration: 0.5-2ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of mouse fibroblast lysates transiently expressing different siRNA in second and third lane (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of mouse fibroblast lysates transiently expressing different siRNA in second and third lane (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008]
Uniprot Description
SETD2: Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Belongs to the histone-lysine methyltransferase family. SET2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: chromosome; nucleoplasm
Molecular Function: histone lysine N-methyltransferase activity (H3-K36 specific); histone-lysine N-methyltransferase activity; protein binding
Biological Process: angiogenesis; embryonic cranial skeleton morphogenesis; forebrain development; mesoderm morphogenesis; mismatch repair; morphogenesis of a branching structure; neural tube closure; pericardium development; regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; stem cell development
Research Articles on SETD2
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Product Notes
The SETD2 setd2 (Catalog #AAA420053) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HYPB/SETD2 (internal region) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's HYPB/SETD2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 290kDa band observed in lysates of mouse fibroblasts. The observed band is knocked down by two different siRNA molecules. Calculated MW of 231kDa according to NP_054878.3). Data obtained from anonymous customer and obtained from a previous batch (from a different goat). The Ponceau red demonstrates proper protein loading. Recommended concentration: 0.5-2ug/ml. Researchers should empirically determine the suitability of the SETD2 setd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ERDPDKQTQN KE. It is sometimes possible for the material contained within the vial of "HYPB/SETD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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