Human Neuropeptide FF (NPFF) ELISA Kit | NPFF elisa kit

Human Neuropeptide FF (NPFF) ELISA Kit

Cat. Num. AAA450932

• Gene Names: NPFF; FMRFAL
• Reactivity: Human
• Synonyms: Neuropeptide FF (NPFF); Human Neuropeptide FF (NPFF) ELISA Kit; FMRFAL Neuropeptide FF-Amide Peptide Precursor Pro-FMRFamide-related neuropeptide FF; NPFF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NPFF. No significant cross-reactivity or interference between NPFF and analogues was observed.
• Sequence Length: 116

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Competitive inhibition
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 4.54pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPFF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPFF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPFF elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NPFF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an qantibody specific to NPFF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NPFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NPFF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NPFF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 998429241
• NCBI GeneID: 8620
• NCBI Accession #: NP_001307225.1
• NCBI GenBank Nucleotide #: NM_001320296.1
• UniProt Accession #: O15130; Q3SXL4
• Molecular Weight: 12,991 Da
• NCBI Official Full Name: pro-FMRFamide-related neuropeptide FF isoform 2
• NCBI Official Synonym Full Names: neuropeptide FF-amide peptide precursor
• NCBI Official Symbol: NPFF
• NCBI Official Synonym Symbols: FMRFAL
• NCBI Protein Information: pro-FMRFamide-related neuropeptide FF
• UniProt Protein Name: Pro-FMRFamide-related neuropeptide FF
• Protein Family: Pro-FMRFamide-related neuropeptide
• UniProt Gene Name: NPFF
• UniProt Synonym Gene Names: NPSF; NPFF; NPAF

NCBI Description

This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

Morphine modulating peptides. Have wide-ranging physiologic effects, including the modulation of morphine-induced analgesia, elevation of arterial blood pressure, and increased somatostatin secretion from the pancreas. Neuropeptide FF potentiates and sensitizes ASIC1 and ASIC3 channels.