SCN11A sirna

SCN11A siRNA (Human)

Cat. Num. AAA8226913

• Gene Names: SCN11A; NaN; PN5; FEPS3; HSAN7; SNS-2; NAV1.9; SCN12A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCN11A; SCN11A siRNA (Human); SCN12A; SNS2; Sodium channel protein type 11 subunit alpha; Peripheral nerve sodium channel 5; PN5; Sensory neuron sodium channel 2; Sodium channel protein type XI subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.9; hNaN; SCN11A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SCN11A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,753

• Applicable Applications for SCN11A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SCN11A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCN11A sirna

siRNA to inhibit SCN11A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 564473389
• NCBI GeneID: 11280
• NCBI Accession #: NP_001274152.1
• NCBI GenBank Nucleotide #: NM_001287223.1
• UniProt Accession #: Q9UI33; Q68K15; Q8NDX3; Q9UHE0; Q9UHM0; A6NN05; C9JD48; C9JR31
• Molecular Weight: 200,415 Da
• NCBI Official Full Name: sodium channel protein type 11 subunit alpha
• NCBI Official Synonym Full Names: sodium channel, voltage gated, type XI alpha subunit
• NCBI Official Symbol: SCN11A
• NCBI Official Synonym Symbols: NaN; PN5; FEPS3; HSAN7; SNS-2; NAV1.9; SCN12A
• NCBI Protein Information: sodium channel protein type 11 subunit alpha
• UniProt Protein Name: Sodium channel protein type 11 subunit alpha
• Protein Family: Sodium channel protein
• UniProt Gene Name: SCN11A
• UniProt Synonym Gene Names: SCN12A; SNS2; PN5
• UniProt Entry Name: SCNBA_HUMAN

NCBI Description

Voltage-gated sodium channels are membrane protein complexes that play a fundamental role in the rising phase of the action potential in most excitable cells. Alpha subunits, such as SCN11A, mediate voltage-dependent gating and conductance, while auxiliary beta subunits regulate the kinetic properties of the channel and facilitate membrane localization of the complex. Aberrant expression patterns or mutations of alpha subunits underlie a number of disorders. Each alpha subunit consists of 4 domains connected by 3 intracellular loops; each domain consists of 6 transmembrane segments and intra- and extracellular linkers.[supplied by OMIM, Apr 2004]

Uniprot Description

SCN11A: This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant sodium channel isoform. Also involved, with the contribution of the receptor tyrosine kinase NTRK2, in rapid BDNF-evoked neuronal depolarization. Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.9/SCN11A subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, sodium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: voltage-gated sodium channel complex; plasma membrane

Molecular Function: voltage-gated sodium channel activity

Biological Process: response to drug; sodium ion transport; generation of action potential; regulation of sensory perception of pain

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type Vii; Episodic Pain Syndrome, Familial, 3